TNFRSF4 antibody

Catalog No. ABIN2749179

Product Details anti-TNFRSF4 Antibody

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The mouse monoclonal antibody Ber-ACT35 (also known as ACT35) recognizes an extracellular epitope of CD134 (TNFRSF4, OX40), an approximately 50 kDa type I transmembrane glycoprotein expressed on activated T cells.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: HTLV 1-transformed HUT-102 cells

Clone: Ber-ACT35

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 3-6 μg/mL.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TNFRSF4 antibody (ABIN2749179)

Voo, Bover, Harline, Vien, Facchinetti, Arima, Kwak, Liu: "Antibodies targeting human OX40 expand effector T cells and block inducible and natural regulatory T cell function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 7, pp. 3641-50, (2013) (PubMed).

Tanijiri, Shimizu, Uehira, Yokoi, Amuro, Sugimoto, Torii, Tajima, Ito, Amakawa, Fukuhara: "Hodgkin's reed-sternberg cell line (KM-H2) promotes a bidirectional differentiation of CD4+CD25+Foxp3+ T cells and CD4+ cytotoxic T lymphocytes from CD4+ naive T cells." in: Journal of leukocyte biology, Vol. 82, Issue 3, pp. 576-84, (2007) (PubMed).

Willett, McMonagle, Ridha, Hosie: "Differential utilization of CD134 as a functional receptor by diverse strains of feline immunodeficiency virus." in: Journal of virology, Vol. 80, Issue 7, pp. 3386-94, (2006) (PubMed).

Li, Zhang: "The effect of anti-human CD134 monoclonal antibody on phytohemagglutinin-induced mRNA expression of perforin in peripheral blood mononuclear cells." in: Cellular & molecular immunology, Vol. 2, Issue 6, pp. 467-71, (2006) (PubMed).

Endl, Rosinger, Schwarz, Friedrich, Rothe, Karges, Schlosser, Eiermann, Schendel, Boehm: "Coexpression of CD25 and OX40 (CD134) receptors delineates autoreactive T-cells in type 1 diabetes." in: Diabetes, Vol. 55, Issue 1, pp. 50-60, (2005) (PubMed).

Aten, Roos, Claessen, Schilder-Tol, Ten Berge, Weening: "Strong and selective glomerular localization of CD134 ligand and TNF receptor-1 in proliferative lupus nephritis." in: Journal of the American Society of Nephrology : JASN, Vol. 11, Issue 8, pp. 1426-38, (2000) (PubMed).

Jones, Fletcher, Pulford, Shahsafaei, Dorfman: "The T-cell activation markers CD30 and OX40/CD134 are expressed in nonoverlapping subsets of peripheral T-cell lymphoma." in: Blood, Vol. 93, Issue 10, pp. 3487-93, (1999) (PubMed).

Target Details for TNFRSF4

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Alternative Name: CD134 (TNFRSF4 Products)

Synonyms: TNFRSF4 antibody, ACT35 antibody, CD134 antibody, Ly-70 antibody, Ox40 antibody, TXGP1L antibody, Txgp1 antibody, OX40 antibody, TNF receptor superfamily member 4 antibody, tumor necrosis factor receptor superfamily, member 4 antibody, TNFRSF4 antibody, Tnfrsf4 antibody

Background: TNF receptor superfamily member 4,CD134 (TNFRSF4, also known as OX40) is a type I transmembrane glycoprotein of TNF/NGF receptor family expressed on activated T cells, fibroblasts, and hematopoietic precursors. Binding to its ligand (OX40L, TNFSF4) on antigen presenting cells gives to the T cell costimulatory signal, and this interaction results also in B cell proliferation and influences T cell memory pool. CD134 is upregulated at sites of inflammation, especially in case of multiple sclerosis and psoriatic lesions.,TNFRSF4, OX40, ACT35, IMD16, TXGP1L

Gene ID: 7293

UniProt: P43489

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints