HACE1 antibody (N-Term)

Catalog No. ABIN2773821

Product Details anti-HACE1 Antibody

Target: HACE1 (HECT Domain and Ankyrin Repeat Containing, E3 Ubiquitin Protein Ligase 1 (HACE1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Dog, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HACE1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RARTVELPED NETAVYTLMP MVMADQHRSV SELLSNSKFD VNYAFGRVKR

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against HACE1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human HACE1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HACE1

Target: HACE1 (HECT Domain and Ankyrin Repeat Containing, E3 Ubiquitin Protein Ligase 1 (HACE1))

Alternative Name: HACE1 (HACE1 Products)

Synonyms: HACE1 antibody, 1700042J16Rik antibody, A730034A22Rik antibody, BC025474 antibody, HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 antibody, HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 L homeolog antibody, HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 antibody, HACE1 antibody, hace1 antibody, hace1.L antibody, Hace1 antibody

Background: HACE1 is a E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. It acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. It acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. HACE1 specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. It may also act as a transcription regulator via its interaction with RARB.
Alias Symbols: -
Protein Interaction Partner: UBE2L3, UBC, OPTN, Unc45b, Flna, Actn1, Cald1, Lima1, Actn4, Tpm3, Tmod3, Coro1c, Vim, Vcl, Tubb5, Tubb4a, Tpm2, Tpm1, Tcp1, Plec, Sqstm1, Npm1, Myh9, Marcks, Hspa9, Hsp90ab1, Hspd1, Hspa8, Fhl1, Csrp1, Cnn2, Cct8, Serpinh1, Capzb, Capza2, Capza1, Actg1,
Protein Size: 694

Molecular Weight: 76 kDa

Gene ID: 57531

NCBI Accession: NM_020771, NP_065822