EIF3I antibody (Middle Region)

Catalog No. ABIN2774409

Product Details anti-EIF3I Antibody

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3I antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RDPSQIDNNE PYMKIPCNDS KITSAVWGPL GECIIAGHES GELNQYSAKS

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against EIF3I. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 325 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF3I

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Alternative Name: EIF3I (EIF3I Products)

Background: EIF3I is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Alias Symbols: EIF3S2, PRO2242, TRIP-1, TRIP1, eIF3-beta, eIF3-p36
Protein Interaction Partner: HUWE1, UBC, SUMO2, SUMO3, RNF2, rev, EIF3G, HECW2, EIF3B, CDK18, TARDBP, NPM1, ICAM1, CD81, IGSF8, PAN2, EIF3D, VCAM1, ITGA4, METTL18, gag-pol, DDX3X, PAXIP1, LIG4, EIF3L, EIF3K, EIF3M, EIF3H, EIF3F, EIF3C, EIF3A, EIF3E, CUL3, CDK2, YWHAZ, SIRT7, ARRB1, R
Protein Size: 325

Molecular Weight: 36 kDa

Gene ID: 8668

NCBI Accession: NM_003757, NP_003748

UniProt: Q13347

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Ribonucleoprotein Complex Subunit Organization, Synthesis of DNA