RNF43 antibody (Ring Finger Protein 43) (Middle Region)

Details for Product anti-RNF43 Antibody No. ABIN2774743, Supplier: Log in to see
Antigen
  • RNF124
  • URCC
  • 4732452J19Rik
  • RGD1305204
  • ring finger protein 43
  • RNF43
  • Rnf43
Epitope
Middle Region
8
5
4
4
3
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
32
9
3
1
1
1
Host
Rabbit
25
8
Clonality
Polyclonal
Conjugate
This RNF43 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
16
14
8
6
4
2
1
1
Options
Supplier
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Supplier Product No.
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human RNF43
Blocking Peptide Blocking peptide for this product available: ABIN983098
Sequence DFDPLVYCSP KGDPQRVDMQ PSVTSRPRSL DSVVPTGETQ VSSHVHYHRH
Predicted Reactivity Cow: 86%, Dog: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against RNF43. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Plasmids, Primers & others Plasmids, Primers & others RNF43 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name RNF43 (RNF43 Antibody Abstract)
Background RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer.
Alias Symbols: DKFZp781H02126, DKFZp781H0392, FLJ20315, MGC125630, RNF124, URCC
Protein Interaction Partner: RSPO1, SFPQ, NONO, HECW1, TP53, UBE2D4, KDM5B, UBE2E3, UBE2N, UBE2E1, UBE2D3, UBE2D2, UBE2D1, AKAP8L, UBC,
Protein Size: 783
Molecular Weight 86 kDa
Gene ID 54894
NCBI Accession NM_017763, NP_060233
UniProt Q68DV7
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 783 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Ring Finger Protein 43 (RNF43) (Middle Region) antibody (ABIN2774743) WB Suggested Anti-RNF43 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Pos...
Product cited in: Sugiura, Yamaguchi, Miyamoto: "A cancer-associated RING finger protein, RNF43, is a ubiquitin ligase that interacts with a nuclear protein, HAP95." in: Experimental cell research, Vol. 314, Issue 7, pp. 1519-28, 2008 (PubMed).

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