RAD18 antibody (Middle Region)
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- Target See all RAD18 Antibodies
- RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))
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Binding Specificity
- Middle Region
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Reactivity
- Human, Rat, Mouse, Rabbit, Cow, Dog, Horse, Guinea Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RAD18 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- LSDRDLKKKL KEHGLSIQGN KQQLIKRHQE FVHMYNAQCD ALHPKSAAEI
- Predicted Reactivity
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
- Characteristics
- This is a rabbit polyclonal antibody against RAD18. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the middle region of human RAD18
- Top Product
- Discover our top product RAD18 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 495 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))
- Alternative Name
- RAD18 (RAD18 Products)
- Synonyms
- RNF73 antibody, 2810024C04Rik antibody, Rad18sc antibody, zgc:55577 antibody, RAD18 antibody, DDBDRAFT_0218120 antibody, DDBDRAFT_0304878 antibody, DDB_0218120 antibody, DDB_0304878 antibody, DKFZp469H0810 antibody, RAD18, E3 ubiquitin protein ligase antibody, RAD18 E3 ubiquitin protein ligase antibody, E3 ubiquitin-protein ligase RAD18 antibody, E3 ubiquitin-protein ligase antibody, DNA repair protein and ATPase antibody, C3HC4-type zinc finger-containing protein antibody, RAD18 antibody, Rad18 antibody, rad18 antibody
- Background
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RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18 is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
Alias Symbols: RNF73
Protein Interaction Partner: TAX1BP1, UBC, SHPRH, RAD18, SIVA1, USP2, UBE2B, UBE2A, HLTF, PCNA, BPLF1, USP5, DBF4, BRCA1, DZIP3, POLH, DTL, Ankrd32, MLKL, ECT2, RPL10A, TRIM23, PCGF6, TRIM8, RFC2, CPD, SPRTN, ZMYM2, MAPK8, POLN, WRNIP1, gag-pol, ORC2, SUMO2, POLK, NBN, Smc6, HIST1H2A
Protein Size: 495 - Molecular Weight
- 56 kDa
- Gene ID
- 56852
- NCBI Accession
- NM_020165, NP_064550
- UniProt
- Q9NS91
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