C-Type Lectin Domain Family 4, Member M (CLEC4M) (N-Term) antibody

Catalog No. ABIN2775392

Product Details

Target: C-Type Lectin Domain Family 4, Member M (CLEC4M)

Binding Specificity: N-Term

Reactivity: Human, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Sequence: MSDSKEPRVQ QLGLLEEDPT TSGIRLFPRD FQFQQIHGHK SSTGCLGHGA

Predicted Reactivity: Human: 100%, Pig: 86%

Characteristics: This is a rabbit polyclonal antibody against CLEC4M. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the n terminal region of human CLEC4M

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 272 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: C-Type Lectin Domain Family 4, Member M (CLEC4M)

Alternative Name: CLEC4M (CLEC4M Products)

Synonyms: CD209L antibody, CD299 antibody, DC-SIGN2 antibody, DC-SIGNR antibody, DCSIGNR antibody, HP10347 antibody, L-SIGN antibody, LSIGN antibody, CD209B antibody, C-type lectin domain family 4 member M antibody, CLEC4M antibody

Background: CLEC4M is a transmembrane receptor and is often referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. It is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses, with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are common and have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 30835, often referred to as DC-SIGN or CD209). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.This gene encodes a type II integral membrane protein that is 77 % identical to CD209 antigen, a HIV gp120-binding protein. This protein, like CD209, efficiently binds both intercellular adhesion molecule 3 (ICAM3) and HIV-1 gp120, and enhances HIV-1 infection of T cells. This gene is mapped to 19p13.3, in a cluster with the CD209 and CD23/FCER2 genes. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Alias Symbols: CD209L, CD299, DC-SIGN2, DC-SIGNR, DCSIGNR, HP10347, L-SIGN, LSIGN, MGC129964, MGC47866
Protein Interaction Partner: TLR2, MME, CLEC4M, CD209, ICAM3, ITGAM, AP2M1, HCVgp1,
Protein Size: 272

Molecular Weight: 30 kDa

Gene ID: 10332

NCBI Accession: NM_214677, NP_999842

UniProt: Q9H2X3