RAG1 antibody (C-Term)

Catalog No. ABIN2775646

Product Details anti-RAG1 Antibody

Target: RAG1 (Recombination Activating Gene 1 (RAG1))

Binding Specificity: C-Term

Reactivity: Mouse, Rat, Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAG1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: IIERDGSIGA WASEGNESGN KLFRRFRKMN ARQSKCYEME DVLKHHWLYT

Predicted Reactivity: Horse: 100%, Mouse: 100%, Pig: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Rag1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1040 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RAG1

Target: RAG1 (Recombination Activating Gene 1 (RAG1))

Alternative Name: Rag1 (RAG1 Products)

Synonyms: RAG-1 antibody, rag1 antibody, RNF74 antibody, Rag-1 antibody, rag-1 antibody, rag1a antibody, recombination activating 1 antibody, recombination activating gene 1 antibody, recombination activating gene 1 S homeolog antibody, Rag1 antibody, RAG1 antibody, rag1 antibody, rag1.S antibody

Background: As a catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities.
Alias Symbols: Rag-1
Protein Interaction Partner: Gmeb1, Sf3a2, Kpna2, Rag1, UBE2C, UBE2H, UBE2E1, UBE2D2, UBE2D1, UBC, CDC34, Trav6-3, Rel, Nfkb1, Rela,
Protein Size: 1040

Molecular Weight: 119 kDa

Gene ID: 19373

NCBI Accession: NM_009019, NP_033045

UniProt: P15919