UPF3B antibody (N-Term)

Catalog No. ABIN2776563

Product Details anti-UPF3B Antibody

Target: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UPF3B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MKEEKEHRPK EKRVTLLTPA GATGSGGGTS GDSSKGEDKQ DRNKEKKEAL

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against UPF3B. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UPF3B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 470 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-UPF3B antibody (ABIN2776563)

Mooney, Jimenez-Mateos, Engel, Mooney, Diviney, Venø, Kjems, Farrell, OBrien, Delanty, Henshall: "RNA sequencing of synaptic and cytoplasmic Upf1-bound transcripts supports contribution of nonsense-mediated decay to epileptogenesis." in: Scientific reports, Vol. 7, pp. 41517, (2017) (PubMed).

Target Details for UPF3B

Target: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Alternative Name: UPF3B (UPF3B Products)

Synonyms: HUPF3B antibody, MRXS14 antibody, RENT3B antibody, UPF3X antibody, 5730594O13Rik antibody, AI317193 antibody, AW541158 antibody, RGD1560264 antibody, UPF3B, regulator of nonsense mediated mRNA decay antibody, UPF3 regulator of nonsense transcripts homolog B (yeast) antibody, UPF3B antibody, Upf3b antibody

Background: UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The protein is one of two functional homologs to yeast Upf3p. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctionsThis gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.
Alias Symbols: UPF3X, HUPF3B, MRXS14, RENT3B
Protein Interaction Partner: NCBP1, CIAO1, STAU1, UBC, UPF1, HECW2, RBM8A, UPF2, SMG1, EIF4A3, WIBG, WBP11, RNPS1, SART1, TOP2A, SNRPA1, NXF1, CAND1, COPS5, CUL5, USP21, EXOSC4, MCRS1, XRN1, TTC19, EIF6, UPF3B, HBB, UPF3A,
Protein Size: 470

Molecular Weight: 52 kDa

Gene ID: 65109

NCBI Accession: NM_023010, NP_075386

UniProt: Q9BZI7