NFIB antibody (Middle Region)

Catalog No. ABIN2777544

Product Details anti-NFIB Antibody

Target: NFIB (Nuclear Factor I/B (NFIB))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Sheep, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIB antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: QDSGQSGSPS HNDPAKNPPG YLEDSFVKSG VFNVSELVRV SRTPITQGTG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against NFIB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFIB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 420 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NFIB antibody (ABIN2777544)

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for NFIB

Target: NFIB (Nuclear Factor I/B (NFIB))

Alternative Name: NFIB (NFIB Products)

Synonyms: NFIB antibody, nf1-b1 antibody, CTF antibody, HMGIC/NFIB antibody, NF-I/B antibody, NF1-B antibody, NFI-B antibody, NFI-RED antibody, NFIB2 antibody, NFIB3 antibody, 6720429L07Rik antibody, E030026I10Rik antibody, nuclear factor I B antibody, nuclear factor 1 B-type antibody, nuclear factor I B L homeolog antibody, nuclear factor I/B antibody, NFIB antibody, LOC100221557 antibody, nfib.L antibody, Nfib antibody

Background: NFIB is a member of the nuclear factor I family of nuclear proteins, which are known to be involved in viral and cellular transcription. NFIB includes the proposed DNA binding and dimerization domain. NFIB recognizes and binds the palindromic sequence 5'-
Alias Symbols: CTF, NF1-B, NFI-B, NFIB2, NFIB3, NF-I/B, NFI-RED, HMGIC/NFIB
Protein Interaction Partner: EED, GRK5, SUMO1, ELAVL1, UBC, SOX2, NFIC, RFX1, NFIX,
Protein Size: 420

Molecular Weight: 47 kDa

Gene ID: 4781

NCBI Accession: NM_005596, NP_005587

UniProt: O00712