NFIX antibody (Middle Region)

Catalog No. ABIN2777545

Product Details anti-NFIX Antibody

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: YFVHTPESGQ SDSSNQQGDA DIKPLPNGHL SFQDCFVTSG VWNVTELVRV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against NFIX. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFIX

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 441 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFIX

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Alternative Name: NFIX (MLZE Products)

Synonyms: MRSHSS antibody, NF1A antibody, SOTOS2 antibody, CTF antibody, NF-I/X antibody, NF1-X antibody, NFI-X antibody, nfix antibody, nuclear factor I/X antibody, nuclear factor I X antibody, nuclear factor 1 X antibody, nuclear factor I/Xa antibody, Nfix antibody, NFIX antibody, Nf1x antibody, nfixa antibody

Background: Nuclear factor I (NFI) proteins constitute a family of sequence-specific transcription factors whose functional diversity is generated through transcription from four different genes (NFI-A, NFI-B, NFI-C, and NFI-X), alternative RNA splicing, and protein heterodimerization. NFI-X has divergent functions after binding in promoter or enhancer position. This property, combined with the differential expression of NFI-X, can achieve cell-type specificity of NFI dependent promoters and enhancers.
Alias Symbols: NF1A
Protein Interaction Partner: QRICH1, NFIX, ZNF614, SUMO1, UBC, NEDD8, JMJD6, ISG15, NFIB, PIR, RFX1, SKI, RB1, HDAC1,
Protein Size: 441

Molecular Weight: 49 kDa

Gene ID: 4784

NCBI Accession: NM_002501, NP_002492

Pathways: Skeletal Muscle Fiber Development