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ALF (N-Term) antibody

Reactivity: Human, Cow, Dog, Guinea Pig, Horse, Rabbit, Rat WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2777620
  • Target
    ALF
    Binding Specificity
    N-Term
    Reactivity
    Human, Cow, Dog, Guinea Pig, Horse, Rabbit, Rat
    Host
    • 2
    • 1
    Rabbit
    Clonality
    • 1
    • 1
    Polyclonal
    Conjugate
    • 3
    Un-conjugated
    Application
    • 3
    • 3
    • 1
    Western Blotting (WB)
    Sequence
    VIPAGRTLPS FTTAELGTSN SSANFTFPGY PIHVPAGVTL QTVSGHLYKV
    Predicted Reactivity
    Cow: 92%, Dog: 92%, Guinea Pig: 83%, Horse: 100%, Human: 100%, Rabbit: 91%, Rat: 100%
    Characteristics
    This is a rabbit polyclonal antibody against ALF. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N terminal region of human ALF
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 453 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    ALF
    Background
    The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involves the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. ALF is a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology.
    Alias Symbols: ALF
    Protein Interaction Partner: GAB1, NKX2-3, ZBTB3, TLX3, MAFF, RBFOX2, HMG20A, PAX9, SIX6, MAFG, HOXC11, HOXC9, HOXA5, TLX2, EMX2, EMX1, CEBPE, ATF4, ASCL3, GTF2A2, SUB1, ID3, CSNK2A2, CSNK2A1, TBP,
    Protein Size: 453
    Molecular Weight
    50 kDa
    Gene ID
    11036
    NCBI Accession
    NM_172196, NP_751946
    UniProt
    Q9UNN4
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