MARCH8 antibody (Middle Region)

Catalog No. ABIN2781250

Product Details anti-MARCH8 Antibody

Target: MARCH8 (Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH8 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: YVQCKVYVQL WKRLKAYNRV IYVQNCPETS KKNIFEKSPL TEPNFENKHG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MARCH8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MARCH8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 291 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MARCH8

Target: MARCH8 (Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8))

Alternative Name: MARCH8 (MARCH8 Products)

Synonyms: MARCH-VIII antibody, MIR antibody, RNF178 antibody, c-MIR antibody, zgc:171495 antibody, 1300017E09Rik antibody, Mir antibody, Mar-08 antibody, MARCH8 antibody, membrane associated ring-CH-type finger 8 antibody, membrane-associated ring finger (C3HC4) 8 antibody, membrane associated ring-CH-type finger 8 L homeolog antibody, MARCH8 antibody, march8 antibody, March8 antibody, march8.L antibody

Background: MARCH8 is an E3 ubiquitin-protein ligase that may regulate immune responses by promoting ubiquitination of MHC-II and CD86, which leads to their subsequent endocytosis and lysosomal degradation. It may also promote ubiquitination and endocytosis of TFRC and FAS. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Alias Symbols: MARCH-VIII, MIR, RNF178, c-MIR
Protein Interaction Partner: TNFRSF10A, UBC, UBE2H, UBE2D1, TFRC, UBE2D3, IL1RAP, IL1R1, CD86,
Protein Size: 291

Molecular Weight: 33 kDa

Gene ID: 220972

NCBI Accession: NM_145021, NP_659458

UniProt: Q5T0T0