HADHB antibody (C-Term)

Catalog No. ABIN2783232

Product Details anti-HADHB Antibody

Target: HADHB (Hydroxyacyl-CoA Dehydrogenase/3-Ketoacyl-CoA Thiolase/enoyl-CoA Hydratase (Trifunctional Protein), beta Subunit (HADHB))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Pig, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HADHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: LLLGPTYATP KVLEKAGLTM NDIDAFEFHE AFSGQILANF KAMDSDWFAE

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Yeast: 83%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against HADHB. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HADHB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 474 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HADHB antibody (ABIN2783232)

Chen, Young, Chatham, Crossman, DellItalia, Shalev: "TXNIP regulates myocardial fatty acid oxidation via miR-33a signaling." in: American journal of physiology. Heart and circulatory physiology, Vol. 311, Issue 1, pp. H64-75, (2016) (PubMed).

Gerin, Clerbaux, Haumont, Lanthier, Das, Burant, Leclercq, MacDougald, Bommer: "Expression of miR-33 from an SREBP2 intron inhibits cholesterol export and fatty acid oxidation." in: The Journal of biological chemistry, Vol. 285, Issue 44, pp. 33652-61, (2010) (PubMed).

Target Details for HADHB

Target: HADHB (Hydroxyacyl-CoA Dehydrogenase/3-Ketoacyl-CoA Thiolase/enoyl-CoA Hydratase (Trifunctional Protein), beta Subunit (HADHB))

Alternative Name: HADHB (HADHB Products)

Synonyms: ECHB antibody, MTPB antibody, TP-BETA antibody, thiolase antibody, fb14g10 antibody, wu:fb14g10 antibody, zgc:56274 antibody, 4930479F15Rik antibody, Mtpb antibody, Hadhb antibody, hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta antibody, hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta S homeolog antibody, hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit antibody, hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit antibody, HADHB antibody, hadhb.S antibody, Hadhb antibody, hadhb antibody

Background: HADHB is the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. Mutations in HADHB gene result in trifunctional protein deficiency. The protein can also bind RNA and decreases the stability of some mRNAs.This gene encodes the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. Mutations in this gene result in trifunctional protein deficiency. The encoded protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. Alternatively spliced transcript variants have been found, however, their full-length nature is not known. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MGC87480, MSTP029, TP-BETA, ECHB, MTPB
Protein Interaction Partner: HUWE1, TP53, TUBG1, SUMO2, SUMO3, UBC, PARK2, PPP6R1, ADRB2, CSNK2A2, PAN2, ATF2, vpu, TIMM8B, COX17, IQCB1, SUOX, CHCHD4, TUBB1, RPS8, OPA1, HSP90AB1, HADHA, DES, GRK5, FBXO6, CDK2, LEO1, RTF1, TK1, SMN1, PSMA3, GRB7, RCC1, CDKN1A, ANXA7, Edc4, MYC, SQST
Protein Size: 474

Molecular Weight: 47 kDa

Gene ID: 3032

NCBI Accession: NM_000183, NP_000174

UniProt: P55084

Pathways: Monocarboxylic Acid Catabolic Process