DPH1 antibody (N-Term)

Catalog No. ABIN2784707

Product Details anti-DPH1 Antibody

Target: DPH1 (DPH1 Homolog (DPH1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rabbit, Rat, Dog, Guinea Pig, Horse, Cow, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: NQIPPEILKN PQLQAAIRVL PSNYNFEIPK TIWRIQQAQA KKVALQMPEG

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 92%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against DPH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DPH1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 443 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DPH1

Target: DPH1 (DPH1 Homolog (DPH1))

Alternative Name: DPH1 (DPH1 Products)

Synonyms: DPH1 antibody, DPH1-OVCA2 antibody, dph1-ovca2 antibody, dph2l antibody, dph2l1 antibody, ovca1 antibody, DPH2L antibody, DPH2L1 antibody, OVCA1 antibody, zgc:110702 antibody, 2310011M22Rik antibody, 4930488F09Rik antibody, AW551873 antibody, Dph2l1 antibody, Ovca1 antibody, RGD1562694 antibody, diphthamide biosynthesis 1 antibody, diphthamide biosynthesis protein 1 antibody, diphthamide biosynthesis 1 L homeolog antibody, 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 antibody, DPH1 antibody, NCU08503 antibody, ATEG_01183 antibody, LELG_00973 antibody, HCAG_03806 antibody, AOR_1_728014 antibody, PTRG_05729 antibody, CTRG_01524 antibody, UREG_06411 antibody, BDBG_05574 antibody, MCYG_04396 antibody, PITG_12187 antibody, MGYG_02224 antibody, LOC100284663 antibody, dph1.L antibody, dph1 antibody, Dph1 antibody, dph-1 antibody

Background: Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).
Alias Symbols: DPH2L, DPH2L1, FLJ33211, OVCA1
Protein Interaction Partner: UBC, TFCP2, MMS19, UBD, TP63, TP73, TP53, RBM8A, HSPA5,
Protein Size: 443

Molecular Weight: 49 kDa

Gene ID: 1801

NCBI Accession: NM_001383, NP_001374

UniProt: Q9BZG8