AGAP2 antibody (Middle Region)

Catalog No. ABIN2784896

Product Details anti-AGAP2 Antibody

Target: AGAP2 (ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Horse, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGAP2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AHARHGPLDT SVEDPQLRSP LHLAAELAHV VITQLLLWYG ADVAARDAQG

Predicted Reactivity: Cow: 100%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CENTG1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CENTG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 836 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for AGAP2

Target: AGAP2 (ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2))

Alternative Name: CENTG1 (AGAP2 Products)

Synonyms: zgc:153779 antibody, CENTG1 antibody, GGAP2 antibody, PIKE antibody, Centg1 antibody, mKIAA0167 antibody, Pike antibody, ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody, AGAP2 antibody, agap2 antibody, Agap2 antibody

Background: CENTG1 is a GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
Alias Symbols: AGAP2, FLJ16430, GGAP2, KIAA0167, PIKE, CENTG1
Protein Interaction Partner: Mprip, Siah1a, Nr4a1, UBC, HOMER1, HOMER2, EPB41L1, PLCG1, PIK3R1, NF2,
Protein Size: 836

Molecular Weight: 90 kDa

Gene ID: 116986

NCBI Accession: NM_014770, NP_055585

UniProt: Q99490