Pseudouridylate Synthase 10 (PUS10) (Middle Region) antibody

Details for Product No. ABIN2785075
Antigen
Epitope
Middle Region
16
8
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
45
27
18
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
45
Clonality
Polyclonal
Conjugate
Un-conjugated
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Western Blotting (WB)
28
19
13
5
3
1
Options
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PUS10
Blocking Peptide Blocking peptide for this product available: ABIN974538
Sequence AVFVAGRYNK YSRNLPQTPW IIDGERKLES SVEELISDHL LAVFKAESFN
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%
Characteristics This is a rabbit polyclonal antibody against CCDC139. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Antigen
Alternative Name PUS10 (PUS10 Antibody Abstract)
Background Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).
Alias Symbols: FLJ32312, MGC126729, MGC126755, DOBI, CCDC139
Protein Size: 529
Molecular Weight 60 kDa
Gene ID 150962
NCBI Accession NM_144709, NP_653310
UniProt Q3MIT2
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 529 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Pseudouridylate Synthase 10 (PUS10) (Middle Region) antibody (ABIN2785075) WB Suggested Anti-PUS10 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positiv...