RFC1 antibody (Middle Region)

Catalog No. ABIN2786753

Product Details anti-RFC1 Antibody

Target: RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Guinea Pig, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFC1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AQAIYASVLP GELMRGYMTQ FPTFPSWLGK HSSTGKHDRI VQDLALHMSL

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against RFC1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RFC1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1147 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RFC1

Target: RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))

Alternative Name: RFC1 (RFC1 Products)

Synonyms: A1 antibody, MHCBFB antibody, PO-GA antibody, RECC1 antibody, RFC antibody, RFC140 antibody, AtRFC1 antibody, replication factor C 1 antibody, replication factor C1 antibody, 140kDa antibody, Alp145 antibody, Recc1 antibody, Replication factor C1 antibody, si:dkey-170e5.1 antibody, zgc:162875 antibody, replication factor C subunit 1 antibody, replication factor C1 antibody, replication factor C (activator 1) 1 antibody, DNA replication factor C complex subunit Rfc1 antibody, RFC1 antibody, Rfc1 antibody, rfc1 antibody

Background: The protein encoded by this gene is the large subunit of replication factor C, which is a five subunit DNA polymerase accessory protein. Replication factor C is a DNA-dependent ATPase that is required for eukaryotic DNA replication and repair. The protein acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It also may have a role in telomere stability.
Alias Symbols: A1, MGC51786, MHCBFB, PO-GA, RECC1, RFC, RFC140
Protein Interaction Partner: SPRTN, UBC, PCNA, RPA3, RPA2, RPA1, YWHAQ, SMC1A, RFC4, NOTCH1, WDR18, RBM14, RFC5, RFC3, RFC2, LAMP2, MYOC, SIRT7, SUMO2, HDGF, RAD17, CSNK2B, tat, SAP25, ZSCAN1, SSBP3, PMS1, CEBPA, BRCA1, RFC1, BRD4, HDAC1, CASP3, TCEAL1, CCND1, RELA, CDK4, CHTF18, SMA
Protein Size: 1147

Molecular Weight: 128 kDa

Gene ID: 5981

NCBI Accession: NM_002913, NP_002904

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA, Dicarboxylic Acid Transport