NDUFA1 antibody (N-Term)

Catalog No. ABIN2789932

Product Details anti-NDUFA1 Antibody

Target: NDUFA1 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Horse, Pig, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDUFA1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VMGVCLLIPG LATAYIHRFT NGGKEKRVAH FGYHWSLMER DRRISGVDRY

Predicted Reactivity: Cow: 85%, Horse: 86%, Human: 100%, Mouse: 92%, Pig: 92%, Rabbit: 79%, Rat: 92%, Sheep: 85%

Characteristics: This is a rabbit polyclonal antibody against NDUFA1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 70 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NDUFA1

Target: NDUFA1 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1))

Alternative Name: NDUFA1 (NDUFA1 Products)

Synonyms: 1810049F12Rik antibody, MWFE antibody, RGD1560955 antibody, CI-MWFE antibody, ZNF183 antibody, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1 antibody, NADH:ubiquinone oxidoreductase subunit A1 antibody, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa antibody, Ndufa1 antibody, NDUFA1 antibody

Background: The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.
Alias Symbols: CI-MWFE, MWFE, ZNF183
Protein Interaction Partner: TRIM63, TRIM55, UBC, GOLGB1,
Protein Size: 70

Molecular Weight: 8 kDa

Gene ID: 4694

NCBI Accession: NM_004541, NP_004532

UniProt: O15239