NFIC antibody (Middle Region)

Catalog No. ABIN2792572

Product Details anti-NFIC Antibody

Target: NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))

Binding Specificity: Middle Region

Reactivity: Human, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIC antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MLAPPPPGLP RLALPPATKP ATTSEGGATS PTSPSYSPPD TSPANRSFVG

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Predicted Reactivity: Dog: 85%, Human: 100%, Pig: 85%

Characteristics: This is a rabbit polyclonal antibody against NFIC. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFIC

Application Details

Application Notes: WB Suggested Anti-NFIC Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: 721_B cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 499 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFIC

Target: NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))

Alternative Name: NFIC (NFIC Products)

Synonyms: LOC100005568 antibody, 1110019L22Rik antibody, 1500041O16Rik antibody, AA589446 antibody, AI746521 antibody, NF1-C antibody, CTF antibody, CTF5 antibody, NF-I antibody, NFI antibody, CNFI-C antibody, nf1-c1 antibody, nuclear factor I/C antibody, nuclear factor I C antibody, nuclear factor I C L homeolog antibody, nfic antibody, NFIC antibody, Nfic antibody, nfic.L antibody

Background: Brg- or hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg1 is the core subunit of Brg-associated factor complexes. BAF complexes can interact with NF1/CTF and RNAP II, and this interaction is closely dependent on the activation of gene transcription.
Alias Symbols: CTF, CTF5, MGC20153, NF-I, NFI
Protein Interaction Partner: HSP90AA1, TFAP4, Cebpb, ELAVL1, SUMO2, UBC, SMURF1, SMAD3, ZCCHC14, ZKSCAN7, TPD52L3, TKTL2, LLPH, NFIB, GLRX, CREBBP, RFX1, TLX1,
Protein Size: 499

Molecular Weight: 55 kDa

Gene ID: 4782

NCBI Accession: NM_205843, NP_995315

UniProt: Q6FI30