NR2C1 antibody (C-Term)

Catalog No. ABIN2792596

This anti-NR2C1 antibody is a Rabbit Polyclonal antibody detecting NR2C1 in WB. Suitable for Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit and Pig.

Quick Overview for NR2C1 antibody (C-Term) (ABIN2792596)

Target: NR2C1 (Nuclear Receptor Subfamily 2, Group C, Member 1 (NR2C1))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR2C1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NR2C1 Antibody

Binding Specificity: C-Term

Sequence: QEKAYVEFQD YITKTYPDDT YRLSRLLLRL PALRLMNATI TEELFFKGLI

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 100%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against NR2C1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NR2C1

Application Details

Application Notes: WB Suggested Anti-NR2C1 Antibody Titration: 2.5 µg/mL
ELISA Titer: 1:312500
Positive Control: HCT15 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 603 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NR2C1

Target: NR2C1 (Nuclear Receptor Subfamily 2, Group C, Member 1 (NR2C1))

Alternative Name: NR2C1

Background: The nuclear orphan receptors NR2C1 represses transcription and binds DNA as a homodimer. NR2C1 binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. NR2C1 may function as a negative modulator to suppress androgen receptor function in prostate cancer. NR2C1 may exert an important repressor in regulating ER activity in mammary glands. The nuclear orphan receptors TR2 (NR2C1) and TR4 form a heterodimer that binds to the epsilon and gamma globin promoter DR1 sites
Alias Symbols: TR2
Protein Interaction Partner: PSPC1, MBD3, SIN3A, RCOR1, KDM1A, POLD3, TRIM28, RBM39, MTA2, MTA1, HDAC3, NR2C2, SMARCA4, SFPQ, RBBP7, RBBP4, NONO, HDAC2, HDAC1, HCFC1, DNMT1, CHD4, NUDT3, UBC, PML, HDAC4, NRIP1, AR, ESR1,
Protein Size: 603

Molecular Weight: 67 kDa

Gene ID: 7181

NCBI Accession: NM_003297, NP_003288

UniProt: Q15625

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway