TCF3 antibody (C-Term)

Catalog No. ABIN2792624

Product Details anti-TCF3 Antibody

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: EENTSAADHS EEEKKELKAP RARTSPDEDE DDLLPPEQKA EREKERRVAN

Cross-Reactivity: Dog (Canine), Chicken, Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Pig: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TCF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TCF3

Application Details

Application Notes: WB Suggested Anti-TCF3 Antibody Titration: 5.0 µg/mL
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 440 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TCF3

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Alternative Name: TCF3 / E2A (TCF3 Products)

Synonyms: E2A antibody, E47 antibody, ITF1 antibody, TCF-3 antibody, VDIR antibody, bHLHb21 antibody, tcf3a antibody, zgc:113045 antibody, A1 antibody, AA408400 antibody, ALF2 antibody, AW209082 antibody, E12 antibody, E12/E47 antibody, ME2 antibody, Pan1 antibody, Pan2 antibody, Tcfe2a antibody, bhlhb21 antibody, e2a antibody, itf1 antibody, tcfe2a antibody, vdir antibody, xtcf-3 antibody, xtcf3 antibody, G12 antibody, TCF7L1 antibody, XE3 antibody, transcription factor 3 antibody, transcription factor 3b antibody, transcription factor 3 L homeolog antibody, transcription factor E2a antibody, TCF3 antibody, tcf3b antibody, Tcf3 antibody, tcf3.L antibody, tcfe2a antibody

Background: TCF3 contains 1 basic helix-loop-helix (bHLH) domain. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. TCF3 binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
Alias Symbols: E2A, ITF1, MGC129647, MGC129648, E47, VDIR, TCF-3, bHLHb21
Protein Interaction Partner: ID3, FAM115A, TLE1, RNF14, UBC, SCX, MAPKAPK2, MAPKAPK3, TCF21, Ube2i, ID2, FUS, CTNNB1, RPL37, MAX, USF1, TCF12, TCF3, MYOD1, Rufy1, Tfap4, Rpa1, Tbx6, Ncl, Twist2, SUPT3H, TRRAP, KAT2A, TADA2A, FOXH1, ELAVL1, TCF24, SETSIP, FLG2, BHLHA15, MYL6B, TIMM50,
Protein Size: 440

Molecular Weight: 48 kDa

Gene ID: 6929

NCBI Accession: NM_001136139

UniProt: P15923

Pathways: WNT Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response