GTF2H1 antibody (Middle Region)

Catalog No. ABIN2792659

Product Details anti-GTF2H1 Antibody

Target: GTF2H1 (General Transcription Factor IIH, Polypeptide 1, 62kDa (GTF2H1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Dog, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2H1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ERFQVTKLCP FQEKIRRQYL STNLVSHIEE MLQTAYNKLH TWQSRRLMKK

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against GTF2H1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GTF2H1

Application Details

Application Notes: WB Suggested Anti-GTF2H1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: ACHN cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 548 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GTF2H1

Target: GTF2H1 (General Transcription Factor IIH, Polypeptide 1, 62kDa (GTF2H1))

Alternative Name: GTF2H1 / BTF2 (GTF2H1 Products)

Synonyms: 62kDa antibody, AW743425 antibody, AW822074 antibody, BTF2 p62 antibody, C77871 antibody, p62 antibody, GTF2H1 antibody, BTF2 antibody, P62 antibody, TFB1 antibody, TFIIH antibody, gtf2h1 antibody, MGC53309 antibody, btf2 antibody, tfiih antibody, CG8151 antibody, Dmel\\CG8151 antibody, Dmp62 antibody, TfBl antibody, l(2)06949 antibody, l(2)06950 antibody, zgc:92126 antibody, general transcription factor IIH subunit 1 antibody, general transcription factor II H, polypeptide 1 antibody, general transcription factor IIH subunit 1 L homeolog antibody, Transcription factor B1 antibody, general transcription factor IIH, polypeptide 1 antibody, GTF2H1 antibody, Gtf2h1 antibody, gtf2h1.L antibody, gtf2h1 antibody, Tfb1 antibody

Background: GTF2H1 is the component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.
Alias Symbols: BTF2, TFIIH, TFB1
Protein Interaction Partner: UBC, CDK7, TP53, RNF11, ERCC5, KLF1, HNF4A, E2F1, TCEA1, POLR2A, GTF2E1, POLR2C, KPNA3, GTF2H2, ACTN1, USHBP1, UVSSA, TRIOBP, TNIP1, REEP5, MMS19, ERCC2, GTF2H4, GTF2H3, ERCC3, RB1, MNAT1, tat, GTF2F1, CTD, RAD23A, XPC, XPA, RPA2, RPA1, RAD23B, ERCC4, ERC
Protein Size: 548

Molecular Weight: 62 kDa

Gene ID: 2965

NCBI Accession: NM_005316, NP_005307

UniProt: P32780

Pathways: DNA Damage Repair