TCEB1 antibody (N-Term)

Catalog No. ABIN2792679

Product Details anti-TCEB1 Antibody

Target: TCEB1 (Transcription Elongation Factor B (SIII), Polypeptide 1 (15kDa, Elongin C) (TCEB1))

Binding Specificity: N-Term

Reactivity: Human, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCEB1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: EHALTSGTIK AMLSGPGQFA ENETNEVNFR EIPSHVLSKV CMYFTYKVRY

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Dog: 100%, Human: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against TCEB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TCEB1

Application Details

Application Notes: WB Suggested Anti-TCEB1 Antibody Titration: 0.2-1 µg/mL
Positive Control: Raji cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 112 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TCEB1

Target: TCEB1 (Transcription Elongation Factor B (SIII), Polypeptide 1 (15kDa, Elongin C) (TCEB1))

Alternative Name: TCEB1 (TCEB1 Products)

Synonyms: 2610043E24Rik antibody, 2610301I15Rik antibody, AA407206 antibody, AI987979 antibody, AW049146 antibody, SIII antibody, eloC antibody, TCEB1 antibody, fb09f09 antibody, wu:fb09f09 antibody, zgc:111990 antibody, zgc:92635 antibody, elongin C antibody, elongin C L homeolog antibody, transcription elongation factor B (SIII), polypeptide 1b antibody, Eloc antibody, eloc.L antibody, ELOC antibody, tceb1b antibody

Background: TCEB1 is known as protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Western blots using two different antibodies (P100962-T200 and P100963_P050 / P100963_P100) against two unique regions of this protein target confirm the same apparent molecular weight in our tests.
Alias Symbols: SIII, eloC
Protein Interaction Partner: UBC, TCEB2, FUS, vif, METTL21C, SOCS4, CUL5, PRAME, POP1, MDM2, VHL, ASB18, ASB12, ASB14, ASB15, ASB9, ASB8, ASB7, ASB5, ASB10, ASB16, ASB13, ASB1, ASB3, APEX1, CUL2, Zswim8, ASB2, CPTP, CBX5, MRAS, JTB, RCAN2, SOCS1, CUL3, WNT7B, NEDD8, HIF1A, EFNB3, CYP
Protein Size: 112

Molecular Weight: 12 kDa

Gene ID: 6921

NCBI Accession: NM_005648, NP_005639

UniProt: Q15369

Pathways: SARS-CoV-2 Protein Interactome