Nucleobindin 1 antibody (C-Term)

Catalog No. ABIN2792699

Product Details anti-Nucleobindin 1 Antibody

Target: Nucleobindin 1 (NUCB1)

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleobindin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PAAHPEGQLK FHPDTDDVPV PAPAGDQKEV DTSEKKLLER LPEVEVPQHL

Cross-Reactivity: Human

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against NUCB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NUCB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 461 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Nucleobindin 1 antibody (ABIN2792699)

Larkin, Costantino, Seaman, Lavoie: "Calnuc Function in Endosomal Sorting of Lysosomal Receptors." in: Traffic (Copenhagen, Denmark), Vol. 17, Issue 4, pp. 416-32, (2016) (PubMed).

Target Details for Nucleobindin 1

Target: Nucleobindin 1 (NUCB1)

Alternative Name: Nucleobindin-1 (NUCB1 Products)

Synonyms: nuc antibody, MGC69294 antibody, MGC81496 antibody, NUCB1 antibody, zgc:153192 antibody, DKFZp459O1814 antibody, B230337F23Rik antibody, C77483 antibody, Calnuc antibody, MTEST82 antibody, Nucb antibody, CALNUC antibody, NUC antibody, nucleobindin 1 antibody, nucleobindin 1 S homeolog antibody, nucb1 antibody, nucb1.S antibody, NUCB1 antibody, Nucb1 antibody

Background: Calnuc or NUCB1 belongs to the nucleobindin family. It is a major calcium-binding protein of the Golgi and is a good Golgi marker. It may be involved in calcium homeostasis. Calnuc also plays roles in regulation of levels of .-amyloid precursor protein (APP) and its proteolytic metabolites to further affect the patho/physiological functions of APP including Alzheimer's disease pathogenesis. Useful for immunohistochemistry and western blot.
Alias Symbols: NUC, CALNUC
Protein Interaction Partner: REL, UBC, MDM2, CTPS2, RAD23B, PRKDC, METTL1, GTF2I, CBS, XPNPEP1, WARS, PTGS2, XPO1, SET, RBL1, MYC, HIVEP1, GNAS, GNAI3, GNAI1, KLC4, PTGS1, NDN, GNAI2, GNAO1, GNAZ,
Protein Size: 461

Molecular Weight: 51 kDa

Gene ID: 4924

NCBI Accession: NM_006184, NP_006175

UniProt: Q02818