CD137 antibody

Catalog No. ABIN2800694

The Mouse Monoclonal anti-CD137 antibody has been validated for ELISA, FACS, Func and IP. It is suitable to detect CD137 in samples from Human and Non-Human Primate. There are 3+ publications available.

Quick Overview for CD137 antibody (ABIN2800694)

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD137 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP)

Clone: 4B4-1

Product Details anti-CD137 Antibody

Purpose: Anti-Hu CD137 Purified Low Endotoxin

Specificity: The mouse monoclonal antibody 4B4-1 recognizes an extracellular conformational epitope on CD137, an approximately 40 kDa type I transmembrane protein of the TNFR family expressed mainly on activated T cells.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Recombinant human CD137 ectodomain

Isotype: IgG1 kappa

Application Details

Application Notes: Functional application: Inhibition of cytokine production.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD137 antibody (ABIN2800694)

Yi, Zhao, Wang, Dai, Hellström, Hellström, Zhang: "Human and mouse CD137 have predominantly different binding CRDs to their respective ligands." in: PLoS ONE, Vol. 9, Issue 1, pp. e86337, (2014) (PubMed).

Fernández Do Porto, Jurado, Pasquinelli, Alvarez, Aspera, Musella, García: "CD137 differentially regulates innate and adaptive immunity against Mycobacterium tuberculosis." in: Immunology and cell biology, Vol. 90, Issue 4, pp. 449-56, (2012) (PubMed).

Chan, Voskens, Lin, Schindler, Azimzadeh, Wang, Taylor, Strome, Schulze: "Epitope mapping of a chimeric CD137 mAb: a necessary step for assessing the biologic relevance of non-human primate models." in: Journal of molecular recognition : JMR, Vol. 22, Issue 3, pp. 242-9, (2009) (PubMed).

Target Details for CD137

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Alternative Name: CD137

Background: TNF receptor superfamily member 9,CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.,TNFRSF9, 41BB, 4-1BB, MGC2172

Gene ID: 3604

UniProt: Q07011

Pathways: Cancer Immune Checkpoints