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HA-Tag antibody

WB, IF, ChIP Host: Rabbit Polyclonal RB0411 unconjugated
Catalog No. ABIN2843192
  • Target See all HA-Tag products
    HA-Tag
    Reactivity
    Please inquire
    Host
    • 74
    • 74
    • 13
    • 7
    • 1
    • 1
    Rabbit
    Clonality
    • 85
    • 82
    Polyclonal
    Conjugate
    • 70
    • 15
    • 13
    • 11
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HA-Tag antibody is un-conjugated
    Application
    • 134
    • 62
    • 39
    • 28
    • 19
    • 16
    • 14
    • 14
    • 9
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)
    Sequence
    YPYDVPDYA
    Purification
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
    Immunogen
    KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
    Clone
    RB0411
    Isotype
    Ig Fraction
  • Application Notes
    IF: 1:10~50. CHIP: 1:100. WB: 1:1000. WB: 1:1000. WB: 1:1000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
    Expiry Date
    6 months
  • Milewska, Kindler, Vkovski, Zeglen, Ochman, Thiel, Rajfur, Pyrc: "APOBEC3-mediated restriction of RNA virus replication." in: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).

    Wu, Sun, Ma, Zhu, Zhang, Pan, Li, Liu, Diao, Li: "A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum." in: Scientific reports, Vol. 6, pp. 27332, (2018) (PubMed).

    Baruah, Hitzman, Zhang, Chaudhuri, Mastej, Wary: "The allosteric glycogen synthase kinase-3 inhibitor NP12 limits myocardial remodeling and promotes angiogenesis in an acute myocardial infarction model." in: The Journal of biological chemistry, Vol. 292, Issue 50, pp. 20785-20798, (2018) (PubMed).

    Simkin, Robin, Giuliano, Vukolic, Moceri, Guy, Wagner, Lacampagne, Allard, Bendahhou: "Andersen's syndrome mutants produce a knockdown of inwardly rectifying K+ channel in mouse skeletal muscle in vivo." in: Cell and tissue research, Vol. 371, Issue 2, pp. 309-323, (2017) (PubMed).

    Di, Wang, Han, Fu, Duerfeldt, Blagg, Mu: "Grp94 Protein Delivers γ-Aminobutyric Acid Type A (GABAA) Receptors to Hrd1 Protein-mediated Endoplasmic Reticulum-associated Degradation." in: The Journal of biological chemistry, Vol. 291, Issue 18, pp. 9526-39, (2016) (PubMed).

    Koo, Yue, Deng, Khuri, Sun: "mTOR Complex 2 Stabilizes Mcl-1 Protein by Suppressing Its Glycogen Synthase Kinase 3-Dependent and SCF-FBXW7-Mediated Degradation." in: Molecular and cellular biology, Vol. 35, Issue 13, pp. 2344-55, (2015) (PubMed).

    Han, Guan, Wang, Hatzoglou, Mu: "L-type Calcium Channel Blockers Enhance Trafficking and Function of Epilepsy-associated ?1(D219N) Subunits of GABAA Receptors." in: ACS chemical biology, (2015) (PubMed).

    Mertz, Tan, Pagala, Bai, Chen, Li, Cho, Shaw, Wang, Peng: "Sequential Elution Interactome Analysis of the Mind Bomb 1 Ubiquitin Ligase Reveals a Novel Role in Dendritic Spine Outgrowth." in: Molecular & cellular proteomics : MCP, Vol. 14, Issue 7, pp. 1898-910, (2015) (PubMed).

    Mizutani, Inoue, Omori, Ito, Tamiya-Koizumi, Takagi, Kojima, Nakamura, Iwaki, Nakatochi, Suzuki, Nozawa, Murate: "Increased acid ceramidase expression depends on upregulation of androgen-dependent deubiquitinases, USP2, in a human prostate cancer cell line, LNCaP." in: Journal of biochemistry, (2015) (PubMed).

    Oh, Yue, Wang, Tong, Chen, Khuri, Sun: "Suppression of death receptor 5 enhances cancer cell invasion and metastasis through activation of caspase-8/TRAF2-mediated signaling." in: Oncotarget, (2015) (PubMed).

    Li, An, Li, Ma, Feng, Zhang, Guo: "DELLA Proteins Interact with FLC to Repress the Flowering Transition." in: Journal of integrative plant biology, (2015) (PubMed).

    Zhao, Yue, Khuri, Sun: "mTOR complex 2 is involved in regulation of Cbl-dependent c-FLIP degradation and sensitivity of TRAIL-induced apoptosis." in: Cancer research, Vol. 73, Issue 6, pp. 1946-57, (2013) (PubMed).

    Oh, Yue, Zhou, Balko, Black, Owonikoko, Khuri, Sun: "Oncogenic Ras and B-Raf proteins positively regulate death receptor 5 expression through co-activation of ERK and JNK signaling." in: The Journal of biological chemistry, Vol. 287, Issue 1, pp. 257-67, (2012) (PubMed).

    Li, Ren, Yue, Chen, Khuri, Sun: "The novel Akt inhibitor API-1 induces c-FLIP degradation and synergizes with TRAIL to augment apoptosis independent of Akt inhibition." in: Cancer prevention research (Philadelphia, Pa.), Vol. 5, Issue 4, pp. 612-20, (2012) (PubMed).

    Zhao, Yue, Lonial, Khuri, Sun: "The NEDD8-activating enzyme inhibitor, MLN4924, cooperates with TRAIL to augment apoptosis through facilitating c-FLIP degradation in head and neck cancer cells." in: Molecular cancer therapeutics, Vol. 10, Issue 12, pp. 2415-25, (2011) (PubMed).

    Fan, Li, Yue, Khuri, Sun: "The eIF4E/eIF4G interaction inhibitor 4EGI-1 augments TRAIL-mediated apoptosis through c-FLIP Down-regulation and DR5 induction independent of inhibition of cap-dependent protein translation." in: Neoplasia (New York, N.Y.), Vol. 12, Issue 4, pp. 346-56, (2010) (PubMed).

    Guo, Wang, Wang, Huang, Liang, Chen: "Dissection of the relationship between RACK1 and heterotrimeric G-proteins in Arabidopsis." in: Plant & cell physiology, Vol. 50, Issue 9, pp. 1681-94, (2009) (PubMed).

    Scarselli, Donaldson: "Constitutive internalization of G protein-coupled receptors and G proteins via clathrin-independent endocytosis." in: The Journal of biological chemistry, Vol. 284, Issue 6, pp. 3577-85, (2009) (PubMed).

    Lin, Liu, Yue, Benbrook, Berlin, Khuri, Sun: "Involvement of c-FLIP and survivin down-regulation in flexible heteroarotinoid-induced apoptosis and enhancement of TRAIL-initiated apoptosis in lung cancer cells." in: Molecular cancer therapeutics, Vol. 7, Issue 11, pp. 3556-65, (2008) (PubMed).

    Wang, Kwak, Zeng, Ellis, Chen, Schiefelbein, Chen: "TRICHOMELESS1 regulates trichome patterning by suppressing GLABRA1 in Arabidopsis." in: Development (Cambridge, England), Vol. 134, Issue 21, pp. 3873-82, (2007) (PubMed).

  • Target
    HA-Tag
    Abstract
    HA-Tag Products
    Target Type
    Tag
    Background
    Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Abgent's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
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