GJA8 antibody (N-Term)

Catalog No. ABIN1882072

Product Details anti-GJA8 Antibody

Target: GJA8 (Gap Junction Protein, alpha 8, 50kDa (GJA8))

Binding Specificity: AA 99-130, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJA8 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Purified Rabbit Polyclonal Antibody (Pab)

Immunogen: This Connexin 50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-130 amino acids from the N-terminal region of human Connexin 50.

Clone: RB1381

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.79 mg/mL

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

References for anti-GJA8 antibody (ABIN1882072)

Ponsaerts, De Vuyst, Retamal, Dhondt, Vermeire, Wang, De Smedt, Zimmermann, Himpens, Vereecke, Leybaert, Bultynck: "Intramolecular loop/tail interactions are essential for connexin 43-hemichannel activity." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 11, pp. 4378-95, (2010) (PubMed).

Shiels, Mackay, Ionides, Berry, Moore, Bhattacharya: "A missense mutation in the human connexin50 gene (GJA8) underlies autosomal dominant "zonular pulverulent" cataract, on chromosome 1q." in: American journal of human genetics, Vol. 62, Issue 3, pp. 526-32, (1998) (PubMed).

Church, Wang, Steele: "The human lens intrinsic membrane protein MP70 (Cx50) gene: clonal analysis and chromosome mapping." in: Current eye research, Vol. 14, Issue 10, pp. 979-81, (1996) (PubMed).

Church, Wang, Steele: "The human lens intrinsic membrane protein MP70 (Cx50) gene: clonal analysis and chromosome mapping." in: Current eye research, Vol. 14, Issue 3, pp. 215-21, (1995) (PubMed).

Target Details for GJA8

Target: GJA8 (Gap Junction Protein, alpha 8, 50kDa (GJA8))

Alternative Name: Connexin 50 (GJA8 Products)

Synonyms: Aey5 antibody, Cnx50 antibody, Cx50 antibody, Lop10 antibody, CAE antibody, CAE1 antibody, CTRCT1 antibody, CX50 antibody, CZP1 antibody, MP70 antibody, Cx45.6 antibody, gap junction protein, alpha 8 antibody, gap junction protein alpha 8 antibody, Gja8 antibody, GJA8 antibody

Background: GJA8 is a an integral membrane protein that belongs to the connexin family, alpha-type (group II) subfamily. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins. This particular connexin only forms junctional channels. GJA8 is expressed in the eye lens, and defects in GJA8 are the cause of zonular pulverulent cataract type 1 (CZP1), a form of autosomal dominant congenital cataract.

Synonyms: GJA8, Gap junction alpha-8 protein, Connexin-50, Lens fiber protein MP70

NCBI Accession: NP_005258

UniProt: P48165