NR5A2 + LRH1 antibody (AA 328-338)

Catalog No. ABIN2851820

This Goat Polyclonal antibody specifically detects NR5A2 + LRH1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for NR5A2 + LRH1 antibody (AA 328-338) (ABIN2851820)

Target: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NR5A2 + LRH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-NR5A2 + LRH1 Antibody

Binding Specificity: AA 328-338

Purpose: NR5A2 / LRH1 (aa328-338)

Sequence: QEQANRSKHE K

Specificity: This antibody is expected to recognize both reported isoforms (NP_995582.1, NP_003813.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 5 μg/mL.

Western Blot: Approx 55 kDa band observed in lysates of cell line HepG2 (calculated MW of 56.5 kDa according to NP_003813.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NR5A2 + LRH1

Target: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Alternative Name: NR5A2

Background: NR5A2, nuclear receptor subfamily 5, group A, member 2, B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2, CYP7A promoter-binding factor, alpha-1-fetoprotein transcription factor, b1-binding factor, hepatocyte transcription factor which activat

Gene ID: 2494

NCBI Accession: NP_995582, NP_003813

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway