WT1 antibody (Center)

Catalog No. ABIN2854288

Product Details anti-WT1 Antibody

Target: WT1 (Wilms Tumor 1 (WT1))

Binding Specificity: Center

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Characteristics: Rabbit Polyclonal antibody to Wilms Tumor 1 (Wilms tumor 1)
Wilms Tumor 1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Wilms Tumor 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*

Comment:

Positive Control: Mouse testis, Rat testis

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.11 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: Wilms Tumor 1 (WT1 Products)

Synonyms: AWT1 antibody, EWS-WT1 antibody, GUD antibody, NPHS4 antibody, WAGR antibody, WIT-2 antibody, WT33 antibody, CWT1 antibody, WT1 antibody, XWT1a antibody, wt-1 antibody, wt1 antibody, xWT1 antibody, WT-1 antibody, gud antibody, wagr antibody, wt33 antibody, xwt1 antibody, wit-2 antibody, si:ch211-160j6.3 antibody, zgc:152697 antibody, D630046I19Rik antibody, Wt-1 antibody, XWT1b antibody, XeWT1 antibody, Wilms tumor 1 antibody, Wilms tumor 1 S homeolog antibody, wilms tumor 1b antibody, Wilms tumor protein antibody, Wilms tumor 1 homolog antibody, Wilms tumor 1 L homeolog antibody, WT1 antibody, wt1.S antibody, wt1 antibody, wt1b antibody, Wt1 antibody, wt1.L antibody

Background: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

Cellular Localization: Nucleus

Molecular Weight: 49 kDa

Gene ID: 7490

Pathways: Tube Formation