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HLA-DRB4 antibody (Center)

HLA-DRB4 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2854374
  • Target See all HLA-DRB4 Antibodies
    HLA-DRB4 (Major Histocompatibility Complex, Class II, DR beta 4 (HLA-DRB4))
    Binding Specificity
    • 14
    • 7
    • 5
    • 2
    • 1
    • 1
    Center
    Reactivity
    • 32
    • 1
    Human
    Host
    • 28
    • 4
    Rabbit
    Clonality
    • 29
    • 3
    Polyclonal
    Conjugate
    • 12
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HLA-DRB4 antibody is un-conjugated
    Application
    • 22
    • 19
    • 3
    • 2
    Western Blotting (WB)
    Characteristics
    Rabbit polyclonal antibody to HLA-DRB4 (major histocompatibility complex, class II, DR beta 4)
    HLA DR beta antibody [N1C3]
    Purification
    Purified by antigen-affinity chromatography.
    Immunogen
    Recombinant protein encompassing a sequence within the center region of human HLA DR beta. The exact sequence is proprietary.
    Isotype
    IgG
    Top Product
    Discover our top product HLA-DRB4 Primary Antibody
  • Application Notes
    Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*
    Comment

    Positive Control: Raji

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target
    HLA-DRB4 (Major Histocompatibility Complex, Class II, DR beta 4 (HLA-DRB4))
    Alternative Name
    HLA-DRB4 (HLA-DRB4 Products)
    Synonyms
    DR-4 antibody, DR4 antibody, DRB4 antibody, HLA-DR4B antibody, major histocompatibility complex, class II, DR beta 4 antibody, HLA-DRB4 antibody
    Background
    HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

    Cellular Localization: Membrane, Single-pass type I membrane protein
    Molecular Weight
    30 kDa
    Gene ID
    3126
    Pathways
    TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response
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