METTL3 antibody

Catalog No. ABIN2854497

Product Details anti-METTL3 Antibody

Target: METTL3 (Methyltransferase Like 3 (METTL3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This METTL3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Rat

Characteristics: Rabbit polyclonal antibody to METTL3 (methyltransferase like 3)
METTL3 antibody [N2C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human METTL3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Molt-4

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-METTL3 antibody (ABIN2854497)

Liang, Guan, Lin, Li, Geng, Li: "METTL3 serves an oncogenic role in human ovarian cancer cells partially via the AKT signaling pathway." in: Oncology letters, Vol. 19, Issue 4, pp. 3197-3204, (2020) (PubMed).

Target Details for METTL3

Target: METTL3 (Methyltransferase Like 3 (METTL3))

Alternative Name: methyltransferase like 3 (METTL3 Products)

Synonyms: fj82g07 antibody, wu:fj82g07 antibody, zgc:77093 antibody, IME4 antibody, M6A antibody, MT-A70 antibody, Spo8 antibody, 2310024F18Rik antibody, methyltransferase like 3 antibody, methyltransferase like 3 L homeolog antibody, methyltransferase-like 3 antibody, mettl3 antibody, mettl3.L antibody, METTL3 antibody, Mettl3 antibody

Background: This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

Cellular Localization: Nucleus speckle

Molecular Weight: 64 kDa

Gene ID: 56339

UniProt: Q86U44