Cofilin 2 antibody (C-Term)

Catalog No. ABIN2854655

Product Details anti-Cofilin 2 Antibody

Target: Cofilin 2 (CFL2)

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit polyclonal antibody to Cofilin 2 (cofilin 2 (muscle))
Cofilin 2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cofilin 2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Raji , Neuro2A , C8D30 , Raw264.7

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Cofilin 2 antibody (ABIN2854655)

Purde, Busch, Kudryashova, Wysocki, Kudryashov: "Oligomerization Affects the Ability of Human Cyclase-Associated Proteins 1 and 2 to Promote Actin Severing by Cofilins." in: International journal of molecular sciences, Vol. 20, Issue 22, (2020) (PubMed).

Target Details for Cofilin 2

Target: Cofilin 2 (CFL2)

Alternative Name: cofilin 2 (CFL2 Products)

Background: Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH -dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN, MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190], Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM]

Cellular Localization: Nucleus matrix , Cytoplasm , cytoskeleton

Molecular Weight: 19 kDa

Gene ID: 1073

UniProt: Q9Y281

Pathways: Caspase Cascade in Apoptosis