PRKG1 antibody (Center)

Catalog No. ABIN2854933

Product Details anti-PRKG1 Antibody

Target: PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))

Binding Specificity: Center

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Characteristics: Rabbit Polyclonal antibody to cGK1 (protein kinase, cGMP-dependent, type I)
cGK1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human cGK1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: NIH-3T3 , JC

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PRKG1

Target: PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))

Alternative Name: cGK1 (PRKG1 Products)

Synonyms: prkg1 antibody, zgc:63955 antibody, Pkgi antibody, cGk1 antibody, 1 antibody, PKG antibody, PRKG1B antibody, PRKGR1B antibody, cGK antibody, cGK 1 antibody, cGK1 antibody, cGKI antibody, cGKI-BETA antibody, cGKI-alpha antibody, CGKI antibody, cGKI-beta antibody, AW125416 antibody, Gm19690 antibody, Prkg1b antibody, Prkgr1b antibody, Prkg1beta antibody, protein kinase, cGMP-dependent, type Ia antibody, protein kinase, cGMP-dependent, type I antibody, protein kinase, cGMP-dependent, type 1 antibody, prkg1a antibody, PRKG1 antibody, prkg1 antibody, Prkg1 antibody

Background: Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]

Molecular Weight: 76 kDa

Gene ID: 5592