GLA antibody

Catalog No. ABIN2854962

Product Details anti-GLA Antibody

Target: GLA (Galactosidase, alpha (GLA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Knockdown/Knockout validation was supported by customer review data.

Cross-Reactivity: Human, Rat

Characteristics: Rabbit Polyclonal antibody to Galactosidase alpha (galactosidase, alpha)
Galactosidase alpha antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human Galactosidase alpha. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa , 293T , Rat lung

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.157 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-GLA antibody (ABIN2854962)

Ivanova, Dao, Kasaci, Adewale, Fikry, Goker-Alpan: "Rapid Clathrin-Mediated Uptake of Recombinant α-Gal-A to Lysosome Activates Autophagy." in: Biomolecules, Vol. 10, Issue 6, (2020) (PubMed).

Hingorani, Metcalf, Deming, Garman, Powers, Gierasch: "Ligand-promoted protein folding by biased kinetic partitioning." in: Nature chemical biology, Vol. 13, Issue 4, pp. 369-371, (2017) (PubMed).

Target Details for GLA

Target: GLA (Galactosidase, alpha (GLA))

Alternative Name: galactosidase alpha (GLA Products)

Synonyms: GALA antibody, Ags antibody, zgc:101584 antibody, MGC130872 antibody, SMU.877 antibody, SCF11.21 antibody, AO090005000217 antibody, alpha-GAL antibody, galactosidase alpha antibody, galactosidase, alpha antibody, galactosidase alpha S homeolog antibody, alpha-galactosidase antibody, aga antibody, alpha-galactosidase A antibody, GLA antibody, Gla antibody, gla antibody, gla.S antibody, agaN antibody, aga antibody, agaL antibody, SCO0541 antibody, rafA antibody, melA antibody, galA antibody, ANI_1_2528074 antibody, ANI_1_1502124 antibody, AOR_1_390174 antibody, CpipJ_CPIJ002066 antibody, MCYG_00962 antibody, MCYG_00791 antibody, Tsp_02909 antibody, Tsp_02508 antibody

Background: This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.

Cellular Localization: Lysosome

Molecular Weight: 49 kDa

Gene ID: 2717

UniProt: P06280

Pathways: SARS-CoV-2 Protein Interactome