Septin 7 antibody

Catalog No. ABIN2855083

Product Details anti-Septin 7 Antibody

Target: Septin 7 (SEPT7)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Septin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm))

Cross-Reactivity: Human, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to SEPT7 (septin 7)
SEPT7 antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SEPT7. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLaS3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.02 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Septin 7

Target: Septin 7 (SEPT7)

Alternative Name: septin 7 (SEPT7 Products)

Synonyms: cdc10 antibody, wu:fb38h06 antibody, zgc:56383 antibody, Septin-7 antibody, CDC10 antibody, CDC3 antibody, NBLA02942 antibody, SEPT7A antibody, Cdc10 antibody, E430034N22 antibody, septin 7a antibody, septin 7 antibody, septin 7 L homeolog antibody, sept7a antibody, SEPT7 antibody, sept7.L antibody, sept7 antibody, Sept7 antibody

Background: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. Although the exact function of this gene has not yet been determined, its high similarity to yeast CDC10 and the high conservative nature of eukaryotic cell cycle machinery suggest a similar role to that of its yeast counterpart. Alternative splicing results in two transcript variants encoding different isoforms.

Cellular Localization: Cytoplasm , Kinetochore , Spindle

Molecular Weight: 51 kDa

Gene ID: 989

UniProt: Q16181