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TUFM antibody

KO Validated Tufm Reactivity: Human WB, IF, IP, IHC (p), ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2855160
  • Target See all TUFM (Tufm) Antibodies
    TUFM (Tufm) (Tu Translation Elongation Factor, Mitochondrial (Tufm))
    Reactivity
    • 59
    • 14
    • 8
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 54
    • 5
    Rabbit
    Clonality
    • 56
    • 3
    Polyclonal
    Conjugate
    • 30
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This TUFM antibody is un-conjugated
    Application
    • 41
    • 22
    • 7
    • 7
    • 4
    • 3
    • 3
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    Specificity
    Knockdown/Knockout validation was supported by references (PMID:26990993)
    Cross-Reactivity
    Human, Mouse
    Characteristics
    Rabbit Polyclonal antibody to TUFM (Tu translation elongation factor, mitochondrial)
    TUFM antibody
    Purification
    Purified by antigen-affinity chromatography.
    Grade
    KO Validated
    Immunogen
    Recombinant protein encompassing a sequence within the center region of human TUFM. The exact sequence is proprietary.
    Isotype
    IgG
  • Application Notes
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
    Comment

    Positive Control: NIH-3T3

    Validation: KO/KD

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.3 mg/mL
    Buffer
    1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target
    TUFM (Tufm) (Tu Translation Elongation Factor, Mitochondrial (Tufm))
    Alternative Name
    Tu translation elongation factor, mitochondrial (Tufm Products)
    Synonyms
    TUFM antibody, D250 antibody, fi06f04 antibody, wu:fi06f04 antibody, zgc:110766 antibody, COXPD4 antibody, EF-TuMT antibody, EFTU antibody, P43 antibody, 2300002G02Rik antibody, C76308 antibody, C76389 antibody, Tu translation elongation factor, mitochondrial antibody, TUFM antibody, tufm antibody, Tufm antibody
    Background
    This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17.

    Cellular Localization: Mitochondrion
    Molecular Weight
    50 kDa
    Gene ID
    7284
    UniProt
    P49411
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