Cofilin antibody

Catalog No. ABIN2855302

Product Details anti-Cofilin Antibody

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Cofilin 1 (cofilin 1 (non-muscle))
Cofilin 1 antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Cofilin antibody (ABIN2855302)

Lee, Du, Hwa, Kim: "Parkin Coordinates Platelet Stress Response in Diabetes Mellitus: A Big Role in a Small Cell." in: International journal of molecular sciences, Vol. 21, Issue 16, (2020) (PubMed).

Chen, Lin, Tsai, He, Luo, Chiu, Herschman, Li: "Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain." in: Stem cells translational medicine, Vol. 9, Issue 4, pp. 499-517, (2020) (PubMed).

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: cofilin 1 (CFL1 Products)

Background: Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH -dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Cellular Localization: Nucleus matrix , Cytoplasm , cytoskeleton

Molecular Weight: 19 kDa

Gene ID: 1072

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events