eEF1A1 antibody

Catalog No. ABIN2855345

Product Details anti-eEF1A1 Antibody

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eEF1A1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Based on a BLAST result, it may also cross with human eEF1A2.

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit polyclonal antibody to EF-1 alpha1 (eukaryotic translation elongation factor 1 alpha 1)
EF-1alpha1 antibody [N1C1]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human eEF1A1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.59 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for eEF1A1

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Alternative Name: eukaryotic translation elongation factor 1 alpha 1 (EEF1A1 Products)

Background: This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66 % of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.

Cellular Localization: Cytoplasm

Molecular Weight: 50 kDa

Gene ID: 1915

UniProt: P68104