Transferrin Receptor antibody

Catalog No. ABIN2855434

Product Details anti-Transferrin Receptor Antibody

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to CD71 (transferrin receptor (p90, CD71))
CD71 antibody [N2C1], Internal

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human CD71. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat , Raji , NCI-H929 , K562 , HL-60 , NIH3T3 , HeLa

Validation: Comparison, KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.47 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Transferrin Receptor antibody (ABIN2855434)

Conde-Rubio, Mylonas, Widmann: "The proteolytic landscape of cells exposed to non-lethal stresses is shaped by executioner caspases." in: Cell death discovery, Vol. 7, Issue 1, pp. 164, (2021) (PubMed).

Singh, Leu, Barnoud, Vonteddu, Gnanapradeepan, Lin, Liu, Barton, Kossenkov, George, Murphy, Dotiwala: "African-centric TP53 variant increases iron accumulation and bacterial pathogenesis but improves response to malaria toxin." in: Nature communications, Vol. 11, Issue 1, pp. 473, (2020) (PubMed).

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: transferrin receptor (TFRC Products)

Synonyms: Trfr antibody, LOC100219280 antibody, tfr antibody, cd71 antibody, tfr1 antibody, trfr antibody, CD71 antibody, T9 antibody, TFR antibody, TFR1 antibody, TR antibody, TRFR antibody, p90 antibody, 2610028K12Rik antibody, AI195355 antibody, AI426448 antibody, AU015758 antibody, E430033M20Rik antibody, Mtvr-1 antibody, Mtvr1 antibody, transferrin receptor antibody, TFRC antibody, Tfrc antibody, tfrc antibody

Background: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.

Cellular Localization: Cell membrane, Single-pass type II membrane protein , Melanosome , Transferrin receptor protein 1 , serum form: Secreted

Molecular Weight: 85 kDa

Gene ID: 7037

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis