Cyclin A antibody

Catalog No. ABIN2855543

Product Details anti-Cyclin A Antibody

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: IP/MS validation was supported by references (PMID:30377384)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Cyclin A2 (cyclin A2)
Cyclin A2 antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Neuro2A , PC-12 , IMR32 , SK-N-AS , HeLa(100 μg/ml Bleomycin treatment for 24 hr)

Validation: IP/MS, KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.73 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Cyclin A antibody (ABIN2855543)

Honda, Inui: "PDZRN3 protects against apoptosis in myoblasts by maintaining cyclin A2 expression." in: Scientific reports, Vol. 10, Issue 1, pp. 1140, (2020) (PubMed).

Ogawa, Yaginuma, Nakatomi, Nakajima, Tada-Shigeyama, Addison, Urata, Matsubara, Watanabe, Matsuo, Sato, Honda, Hikiji, Watanabe, Kokabu: "Transducin-like enhancer of split 3 regulates proliferation of melanoma cells via histone deacetylase activity." in: Oncotarget, Vol. 10, Issue 3, pp. 404-414, (2019) (PubMed).

Fu, Xu, Chen, Li, Zhao, Chen: "Microarray analysis reveals Tmub1 as a cell cycle-associated protein in rat hepatocytes." in: Molecular medicine reports, Vol. 17, Issue 3, pp. 4337-4344, (2018) (PubMed).

Li, Wu, Lee, Li, Hsieh, Ho, Ho, Chen, Wang, Yen, Yang, Chen: "Hinokitiol induces DNA damage and autophagy followed by cell cycle arrest and senescence in gefitinib-resistant lung adenocarcinoma cells." in: PLoS ONE, Vol. 9, Issue 8, pp. e104203, (2015) (PubMed).

Lin, Chou, Lee, Chen, Fong, Chang, Huang, Wu, Chang, Wang, Chiu: "The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB." in: Cancer cell international, Vol. 14, Issue 1, pp. 1, (2014) (PubMed).

Target Details for Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Alternative Name: cyclin A2 (CCNA2 Products)

Synonyms: CCN1 antibody, CCNA antibody, AA408589 antibody, Ccn-1 antibody, Ccn1 antibody, Ccna antibody, CycA2 antibody, Cyca antibody, cb671 antibody, chunp6924 antibody, wu:fi36f03 antibody, CYCA antibody, Cyclin-A2 antibody, ccna2 antibody, ccn1 antibody, ccna antibody, ccna1 antibody, Cyclin-A antibody, cyclinA antibody, cyclin A2 antibody, cyclin A2 S homeolog antibody, cyclin A2 L homeolog antibody, CCNA2 antibody, Ccna2 antibody, ccna2 antibody, ccna2.S antibody, ccna2.L antibody

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Cellular Localization: Nucleus , Cytoplasm

Molecular Weight: 49 kDa

Gene ID: 890

UniProt: P20248

Pathways: PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA