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GPC1 antibody

KO Validated GPC1 Reactivity: Human WB, ELISA, IP, ICC, IF, IHC (p), FACS Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2855849
  • Target See all GPC1 Antibodies
    GPC1 (Glypican 1 (GPC1))
    Reactivity
    • 45
    • 19
    • 14
    Human
    Host
    • 57
    • 8
    • 2
    Rabbit
    Clonality
    • 59
    • 8
    Polyclonal
    Conjugate
    • 26
    • 10
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This GPC1 antibody is un-conjugated
    Application
    • 31
    • 26
    • 26
    • 23
    • 14
    • 7
    • 7
    • 7
    • 6
    • 4
    • 4
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
    Cross-Reactivity
    Human, Mouse, Rat
    Characteristics
    Rabbit Polyclonal antibody to Glypican 1 (glypican 1)
    Glypican 1 antibody [N3C3]
    Purification
    Purified by antigen-affinity chromatography.
    Grade
    KO Validated
    Immunogen
    Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.
    Isotype
    IgG
  • Application Notes
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. ELISA: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
    Comment

    Positive Control: MDA-MB-231 , MDA-MB-231 (24 μg/ml Tunicamycin for 16hr) , U87-MG membrane extracts , U87-MG conditioned medium , Mouse brain , mouse fetal brain , A431 , MCF-7 , AsPC-1

    Validation: Comparison, KO/KD, Orthogonal

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.58 mg/mL
    Buffer
    1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Lu, Niu, Liu, Gao, Sun, Zhao: "Elevated glypican-1 expression is associated with an unfavorable prognosis in pancreatic ductal adenocarcinoma." in: Cancer medicine, Vol. 6, Issue 6, pp. 1181-1191, (2018) (PubMed).

    Huang, Ge, Izzi, Greenspan: "α3 Chains of type V collagen regulate breast tumour growth via glypican-1." in: Nature communications, Vol. 8, pp. 14351, (2018) (PubMed).

  • Target
    GPC1 (Glypican 1 (GPC1))
    Alternative Name
    glypican 1 (GPC1 Products)
    Synonyms
    gpc1 antibody, zgc:63947 antibody, GPC1 antibody, Glypican-1 antibody, glypican antibody, AI462976 antibody, HSPG M12 antibody, HSPGM12 antibody, glypican 1b antibody, glypican 1 antibody, gpc1b antibody, GPC1 antibody, gpc1 antibody, Gpc1 antibody
    Background
    Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

    Molecular Weight
    62 kDa
    Gene ID
    2817
    UniProt
    P35052
    Pathways
    Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation
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