Dystroglycan antibody

Catalog No. ABIN2856020

Product Details anti-Dystroglycan Antibody

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dystroglycan antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Cross-Reactivity: Horse, Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to alpha Dystroglycan (dystroglycan 1 (dystrophin-associated glycoprotein 1))
DAG1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DAG1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG membrane fraction extract , MCF-7 membrane extracts , 293T membrane extracts

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.21 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Dystroglycan antibody (ABIN2856020)

Jae, Raaben, Herbert, Kuehne, Wirchnianski, Soh, Stubbs, Janssen, Damme, Saftig, Whelan, Dye, Brummelkamp: "Virus entry. Lassa virus entry requires a trigger-induced receptor switch." in: Science (New York, N.Y.), Vol. 344, Issue 6191, pp. 1506-10, (2014) (PubMed).

Target Details for Dystroglycan

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Alternative Name: dystroglycan 1 (DAG1 Products)

Synonyms: LOC398500 antibody, dag1 antibody, MGC53537 antibody, 156DAG antibody, A3a antibody, AGRNR antibody, DAG antibody, MDDGC7 antibody, MDDGC9 antibody, DAG1 antibody, RAB7 antibody, dg antibody, a3a antibody, dag antibody, agrnr antibody, 156dag antibody, D9Wsu13e antibody, DG antibody, Dp427 antibody, Dp71 antibody, CG18250 antibody, CT41273 antibody, DmDG antibody, Dmel\\CG18250 antibody, atu antibody, dgn antibody, dys antibody, GB14967 antibody, APOJ antibody, CLI antibody, RATTRPM2B antibody, SGP-2 antibody, SGP2 antibody, SP-40 antibody, SP40 antibody, TRPM-2 antibody, TRPM2B antibody, Trpm2 antibody, Trpmb antibody, Ala-1 antibody, H9/25 antibody, Ly-27 antibody, Ly-6 antibody, Ly27 antibody, wu:fb83d06 antibody, wu:fi25f06 antibody, wu:fi37b08 antibody, zgc:109786 antibody, DystroGlycaN antibody, dystroglycan 1 L homeolog antibody, dystroglycan 1 antibody, dystroglycan 1 S homeolog antibody, Dystroglycan antibody, dystroglycan antibody, clusterin antibody, lymphocyte antigen 6 complex antibody, dgn-1 antibody, dag1.L antibody, dgn-2 antibody, dgn-3 antibody, DAG1 antibody, dag1.S antibody, Dag1 antibody, dag1 antibody, Dg antibody, LOC408826 antibody, Clu antibody, Ly6 antibody

Background: Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence.

Cellular Localization: Alpha-dystroglycan: Secreted , extracellular space , Beta-dystroglycan: Cell membrane , Cytoplasm , cytoskeleton

Molecular Weight: 97 kDa

Gene ID: 1605

UniProt: Q14118

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus