PPP1CC antibody (Center)

Catalog No. ABIN2856137

Product Details anti-PPP1CC Antibody

Target: PPP1CC (Protein Phosphatase 1, Catalytic Subunit, gamma Isoform (PPP1CC))

Binding Specificity: Center

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1CC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Cross-Reactivity: Rat (Rattus), Xenopus laevis, Xenopus tropicalis, Chicken, Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Rat (100 %), Xenopus laevis (100 %), Xenopus Tropicalis (100 %), Chicken (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to PP1C gamma (protein phosphatase 1, catalytic subunit, gamma isozyme)
PP1C gamma antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PP1C gamma. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-PPP1CC antibody (ABIN2856137)

Kenney, Genheden, Moon, Wang, Foster, Proud: "Eukaryotic elongation factor 2 kinase regulates the synthesis of microtubule-related proteins in neurons." in: Journal of neurochemistry, Vol. 136, Issue 2, pp. 276-84, (2016) (PubMed).

Wei, Ngo, Wu, Lee: "Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint." in: Molecular biology of the cell, Vol. 22, Issue 19, pp. 3584-94, (2012) (PubMed).

Target Details for PPP1CC

Target: PPP1CC (Protein Phosphatase 1, Catalytic Subunit, gamma Isoform (PPP1CC))

Alternative Name: PP1C gamma (PPP1CC Products)

Synonyms: PP-1G antibody, ppp1g antibody, PP1 antibody, dis2m1 antibody, fe06g11 antibody, fi22e08 antibody, wu:fe06g11 antibody, wu:fi22e08 antibody, PP1C antibody, PPP1G antibody, Ppp1cc1 antibody, ppp1cc-a antibody, protein phosphatase 1, catalytic subunit, gamma isozyme antibody, protein phosphatase 1, catalytic subunit, gamma isoform antibody, protein phosphatase 1 catalytic subunit gamma antibody, protein phosphatase 1, catalytic subunit, gamma isozyme L homeolog antibody, ppp1cc antibody, Ppp1cc antibody, PPP1CC antibody, ppp1cc.L antibody

Background: Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II.

Molecular Weight: 37 kDa

Gene ID: 5501

Pathways: Cellular Glucan Metabolic Process, Lipid Metabolism