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AKR1C1 antibody

KO Validated DDH Reactivity: Human WB, IHC, IHC (p), IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2856139
  • Target See all AKR1C1 (DDH) Antibodies
    AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))
    Reactivity
    • 34
    • 12
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 28
    • 6
    Rabbit
    Clonality
    • 31
    • 3
    Polyclonal
    Conjugate
    • 23
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This AKR1C1 antibody is un-conjugated
    Application
    • 21
    • 11
    • 10
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Cross-Reactivity
    Human, Mouse
    Characteristics
    Rabbit Polyclonal antibody to AKR1C1 (aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1, 20-alpha (3-alpha)-hydroxysteroid dehydrogenase))
    AKR1C1 antibody
    Purification
    Purified by antigen-affinity chromatography.
    Grade
    KO Validated
    Immunogen
    Recombinant protein encompassing a sequence within the center region of human AKR1C1. The exact sequence is proprietary.
    Isotype
    IgG
  • Application Notes
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
    Comment

    Positive Control: mouse liver , A549 , AKR1C1-transfected 293T

    Validation: KO/KD, Orthogonal, Overexpression

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.6 mg/mL
    Buffer
    1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Zhu, Hu, Zeng, Chang, Ge, Wang, Yan, Zhao, Cao, Ying, Gu, Zheng, He, Yang: "The SIRT2-mediated deacetylation of AKR1C1 is required for suppressing its pro-metastasis function in Non-Small Cell Lung Cancer." in: Theranostics, Vol. 10, Issue 5, pp. 2188-2200, (2020) (PubMed).

    Zeng, Zhu, You, Dong, Yang, Zhu, He: "Liquiritin, as a Natural Inhibitor of AKR1C1, Could Interfere With the Progesterone Metabolism." in: Frontiers in physiology, Vol. 10, pp. 833, (2019) (PubMed).

    Zhu, Chang, Yan, Hu, Zeng, Zhou, Yuan, Ying, Cao, He, Yang: "AKR1C1 Activates STAT3 to Promote the Metastasis of Non-Small Cell Lung Cancer." in: Theranostics, Vol. 8, Issue 3, pp. 676-692, (2018) (PubMed).

  • Target
    AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))
    Alternative Name
    aldo-keto reductase family 1 member C1 (DDH Products)
    Synonyms
    2-ALPHA-HSD antibody, 20-ALPHA-HSD antibody, C9 antibody, DD1 antibody, DD1/DD2 antibody, DDH antibody, DDH1 antibody, H-37 antibody, HAKRC antibody, HBAB antibody, MBAB antibody, aldo-keto reductase family 1 member C1 antibody, aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase) antibody, AKR1C1 antibody
    Background
    This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

    Cellular Localization: Cytoplasm
    Molecular Weight
    37 kDa
    Gene ID
    1645
    UniProt
    Q04828
    Pathways
    Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process
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