VPS16 antibody (C-Term)

Catalog No. ABIN2856527

Product Details anti-VPS16 Antibody

Target: VPS16 (Vacuolar Protein Sorting-Associated Protein 16 Homolog (VPS16))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS16 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit polyclonal antibody to VPS16 (vacuolar protein sorting 16 homolog (S. cerevisiae))
VPS16 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS16. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: HepG2 , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.75 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for VPS16

Target: VPS16 (Vacuolar Protein Sorting-Associated Protein 16 Homolog (VPS16))

Alternative Name: VPS16 (VPS16 Products)

Background: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.

Cellular Localization: Late endosome membrane, Peripheral membrane protein, Cytoplasmic side , Lysosome membrane, Peripheral membrane protein, Cytoplasmic side

Molecular Weight: 95 kDa

Gene ID: 64601