RANKL antibody (C-Term)
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- Target See all RANKL (TNFSF11) Antibodies
- RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
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Binding Specificity
- C-Term
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Reactivity
- Human, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RANKL antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Cow (Bovine), Chicken, Mouse (Murine)
- Cross-Reactivity (Details)
- Mouse (93 %), Chicken (87 %), Bovine (100 %)
- Characteristics
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Rabbit Polyclonal antibody to RANKL (tumor necrosis factor (ligand) superfamily, member 11)
RANKL antibody [C2C3], C-term - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RANKL. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product TNFSF11 Primary Antibody
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- Application Notes
- Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- Comment
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Positive Control: A431 , H1299 , HeLa , HepG2 , Rat lung
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
- Alternative Name
- RANKL (TNFSF11 Products)
- Background
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This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
Cellular Localization: Isoform 1: Cell membrane, Single-pass type II membrane protein , Isoform 3: Cell membrane, Single-pass type II membrane protein , Isoform 2: Cytoplasm , Tumor necrosis factor ligand superfamily member 11 , soluble form: Secreted - Molecular Weight
- 35 kDa
- Gene ID
- 8600
- Pathways
- NF-kappaB Signaling
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