Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

X-Ray Repair Cross Complementing 5 antibody

Reactivity: Human WB, IP, ICC, IF, IHC (p), PLA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2856716
  • Target
    X-Ray Repair Cross Complementing 5
    Reactivity
    • 7
    • 2
    Human
    Host
    • 5
    • 3
    Rabbit
    Clonality
    • 5
    • 3
    Polyclonal
    Conjugate
    • 8
    Un-conjugated
    Application
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA)
    Cross-Reactivity
    Human, Mouse, Rat
    Characteristics
    Rabbit Polyclonal antibody to Ku80 (X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining))
    Ku80 antibody [N3C2], Internal
    Purification
    Purified by antigen-affinity chromatography.
    Immunogen
    Recombinant protein encompassing a sequence within the center region of human Ku80. The exact sequence is proprietary.
    Isotype
    IgG
  • Application Notes
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
    Comment

    Positive Control: Neuro2A , PC-12 , Rat2

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.41 mg/mL
    Buffer
    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Chen, Lin, Liang, Villalobos, Villaflores, Lou, Lai, Hsiao: "UVB Irradiation Induced Cell Damage and Early Onset of Junbb Expression in Zebrafish." in: Animals : an open access journal from MDPI, Vol. 10, Issue 6, (2020) (PubMed).

  • Target
    X-Ray Repair Cross Complementing 5
    Synonyms
    KARP-1 antibody, KARP1 antibody, KU80 antibody, KUB2 antibody, Ku86 antibody, NFIV antibody, AI314015 antibody, Ku80 antibody, X-ray repair cross complementing 5 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 antibody, XRCC5 antibody, Xrcc5 antibody
    Background
    The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

    Cellular Localization: Nucleus
    Molecular Weight
    83 kDa
    Gene ID
    7520
    UniProt
    P13010
You are here:
Support