DAZ2 antibody (Deleted in Azoospermia 2) (Center)

Details for Product anti-DAZ2 Antibody No. ABIN2856724
Antigen
Epitope
Center
10
10
8
6
2
2
1
1
1
Reactivity
Human
37
7
7
1
1
1
1
1
1
Host
Rabbit
37
Clonality
Polyclonal
Conjugate
This DAZ2 antibody is un-conjugated
4
4
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
36
16
5
2
2
2
1
1
Options
Immunogen Recombinant protein encompassing a sequence within the center region of human DAZ2. The exact sequence is proprietary.
Isotype IgG
Characteristics Rabbit polyclonal antibody to DAZ2 (deleted in azoospermia 2)
DAZ2 antibody [N1N3]
Purification Purified by antigen-affinity chromatography.
Antigen
Alternative Name DAZ2 (DAZ2 Antibody Abstract)
Background This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications, one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat, the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified, this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains one copy of the 10.8 kb repeat. Alternative splicing results in multiple transcript variants encoding different isoforms.

Cellular Localization: Cytoplasm , Nucleus
Molecular Weight 63 kDa
Gene ID 57055
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HeLa , Molt-4 , Raji

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Deleted in Azoospermia 2 (DAZ2) (Center) antibody (ABIN2856724) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DAZ2, ...
Image no. 2 for anti-Deleted in Azoospermia 2 (DAZ2) (Center) antibody (ABIN2856724) WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 7.5% SDS PAGE antibody dilut...