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AIF1 antibody (Allograft Inflammatory Factor 1) (C-Term) Primary Antibody

AIF1 Reactivity: Human, Mouse, Rat IHC (fro), IHC (p), IHC, WB Host: Rabbit Polyclonal unconjugated
Pubmed (13) Independent Validation (1)
Catalog No. ABIN2857032
$509.08
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100 μL
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  • Target
    AIF1
    Binding Specificity
    • 21
    • 16
    • 13
    • 12
    • 11
    • 10
    • 9
    • 6
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 107
    • 54
    • 48
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 81
    • 32
    • 15
    • 1
    Rabbit
    Clonality
    • 94
    • 35
    Polyclonal
    Conjugate
    • 55
    • 20
    • 13
    • 9
    • 5
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This AIF1 antibody is un-conjugated
    Application
    • 84
    • 64
    • 57
    • 23
    • 20
    • 14
    • 14
    • 9
    • 9
    • 7
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
    Cross-Reactivity
    Cow (Bovine), Pig (Porcine), Rhesus Monkey
    Cross-Reactivity (Details)
    Pig (100 %), Rhesus Monkey (100 %), Bovine (92 %)
    Characteristics
    Rabbit Polyclonal antibody to Iba1 (allograft inflammatory factor 1)
    Iba1 antibody
    Purification
    Purified by antigen-affinity chromatography.
    Immunogen
    Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.
    Isotype
    IgG
  • Application Notes
    Suggested dilution Reference Immunohistochemistry Assay-dependent dilution IHC (Frozen sections) Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunohistochemistryAssay-dependent dilution IHC (Frozen sections)Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
    Comment

    Positive Control: Molt-4 , THP-1 , HL-60 , *Mouse brain

    Restrictions
    For Research Use only
  • Validation #101924 (Immunofluorescence)
    'Independent Validation' Badge
    by
    Centre d'Immunologie de Marseille-Luminy
    No.
    #101924
    Date
    10/19/2017
    Antigen
    AIF1
    Lot Number
    39476
    Method validated
    Immunofluorescence
    Positive Control
    PFA fixed tissue section brain of CX3CR1 GFP mouse. Staining with Iba1 (Wako, 019-19741) and Donkey anti-rabbit Fab’2 AlexaFluor 647
    Negative Control
    Notes
    Passed. Immunostaining of mouse brain samples with ABIN2857032 shows specifically the expected staining pattern.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN2857032
    Secondary Antibody
    donkey Fab’2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806)
    Full Protocol
    • Harvest Brain from 13 weeks old mice in 1x Dulbecco’s phosphate buffered saline (DPBS) (Gibco Life Technologies, 14200-067).
    • Fix mouse brain in antigen fix (Diapath, P0014) for 2h at RT.
    • Wash tissue in 0.1M pH7.4 phosphate buffer at for 1h at 4°C.
    • Dehydrate tissue in 30% sucrose solution ON at 4°C.
    • Snap freeze tissue in Tissue Freezing Medium (ElectroMicroscopy Science, 72592-C) at -80°C.
    • Cut blocks into 20µm sections using a cryostat (Leica, CM3050 S).
    • Transfer sections to a slide.
    • Create a hydrophobic barrier on the slide around sections with Dako pen (Dako, S2002, lot 00081640).
    • Place slide in a humidified chamber and rehydrate sections in 0.1M TrisHCl pH7.4 for 10min at RT.
    • Gently remove buffer by tapping slide.
    • Permeabilize tissue in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA, for 20min at RT.
    • Incubate sections with primary AIF1 (c-term) (antibodies-online, ABIN2857032, Lot 39476) diluted 1:500 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA for 5h at RT. Incubate the negative contrls sections with diluted rabbit serum.
    • Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
    • Incubate sections with secondary donkey Fab’2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806) diluted 1:500 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 and 0.5% BSA ON at RT.
    • Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
    • Add approximately 10μl of Slowfade® Gold antifade reagent (Life technologies, 536937, lot 1226836) for each section and mount cover slip.
    • Image acquisition on a LSM 880 (Zeiss), 20x magnification, 1000 resolution.
    Experimental Notes
    Microglial cells in the brain of the CX3CR1 GFP mouse expressing GFP are known to express Aif-1. Immunostaining of the PFA fixed tissue sections with ABIN2857032 showed the expected co-localization of the GFP and Aif-1 signals.
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Kaehler, Seitter, Sandbichler, Tschugg, Obermair, Stefanova, Koschak: "Assessment of the Retina of Plp-α-Syn Mice as a Model for Studying Synuclein-Dependent Diseases." in: Investigative ophthalmology & visual science, Vol. 61, Issue 6, pp. 12, 2020 (PubMed).

    Wahle, Sofranko, Dekkers, Miller, Heusinkveld, Albrecht, Cassee, Schins: "Evaluation of neurological effects of cerium dioxide nanoparticles doped with different amounts of zirconium following inhalation exposure in mouse models of Alzheimer's and vascular disease." in: Neurochemistry international, Vol. 138, pp. 104755, 2020 (PubMed).

    Li, Zhang, Yang, Blevins, Norris, Zhao, Huang: "C-terminal binding proteins 1 and 2 in traumatic brain injury-induced inflammation and their inhibition as an approach for anti-inflammatory treatment." in: International journal of biological sciences, Vol. 16, Issue 7, pp. 1107-1120, 2020 (PubMed).

    Mondor, Baratin, Lagueyrie, Saro, Henri, Gentek, Suerinck, Kastenmuller, Jiang, Bajénoff: "Lymphatic Endothelial Cells Are Essential Components of the Subcapsular Sinus Macrophage Niche." in: Immunity, Vol. 50, Issue 6, pp. 1453-1466.e4, 2019 (PubMed).

    Olechnowicz, Weivoda, Lwin, Leung, Gooding, Nador, Javaid, Ramasamy, Rao, Edwards, Edwards: "Multiple myeloma increases nerve growth factor and other pain-related markers through interactions with the bone microenvironment." in: Scientific reports, Vol. 9, Issue 1, pp. 14189, 2019 (PubMed).

    Wahl, Solon-Biet, Wang, Wali, Pulpitel, Clark, Raubenheimer, Senior, Sinclair, Cooney, de Cabo, Cogger, Simpson, Le Couteur: "Comparing the Effects of Low-Protein and High-Carbohydrate Diets and Caloric Restriction on Brain Aging in Mice." in: Cell reports, Vol. 25, Issue 8, pp. 2234-2243.e6, 2019 (PubMed).

    Huang, Lin, Chen, Chen, Chen, Hsu, Wu: "NADPH oxidases as potential pharmacological targets against increased seizure susceptibility after systemic inflammation." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 140, 2019 (PubMed).

    Götzl, Brendel, Werner, Parhizkar, Sebastian Monasor, Kleinberger, Colombo, Deussing, Wagner, Winkelmann, Diehl-Schmid, Levin, Fellerer, Reifschneider, Bultmann, Bartenstein, Rominger, Tahirovic et al.: "Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism. ..." in: EMBO molecular medicine, Vol. 11, Issue 6, 2019 (PubMed).

    Lin, Hsuan, Lin, Kuo, Lin, Su, Niu, Chang, Lin: "Human Umbilical Cord Mesenchymal Stem Cells Preserve Adult Newborn Neurons and Reduce Neurological Injury after Cerebral Ischemia by Reducing the Number of Hypertrophic Microglia/Macrophages." in: Cell transplantation, Vol. 26, Issue 11, pp. 1798-1810, 2018 (PubMed).

    Zha, Liu, Zhu, Liu, Lu, Xu, Yu, Liu: "A scFv antibody targeting common oligomeric epitope has potential for treating several amyloidoses." in: Scientific reports, Vol. 6, pp. 36631, 2018 (PubMed).

    Zera, Zastre: "Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes." in: PLoS ONE, Vol. 12, Issue 10, pp. e0186707, 2017 (PubMed).

    Liu, Shi, Huang, Peterson, Wu, Lan, Zhang, He, Woods, Du, Wu, Wang: "Astragaloside IV inhibits microglia activation via glucocorticoid receptor mediated signaling pathway." in: Scientific reports, Vol. 6, pp. 19137, 2016 (PubMed).

    Wolf, Damme, Stroobants, DHooge, Beck, Hermans-Borgmeyer, Lüllmann-Rauch, Dierks, Lübke: "A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease." in: Disease models & mechanisms, Vol. 9, Issue 9, pp. 1015-28, 2016 (PubMed).

  • Target
    AIF1
    Alternative Name
    Iba1 (AIF1 Antibody Abstract)
    Synonyms
    AIF-1, IBA1, IRT-1, IRT1, AI607846, D17H6S50E, G1, Iba1, Bart1, iba1, mrf-1, fa04b11, wu:fa04b11, AIF, AIF1, aif1, allograft inflammatory factor 1, AIF1, Aif1, aif1
    Background
    This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene.

    Molecular Weight
    17 kDa
    Gene ID
    199
    Pathways
    Smooth Muscle Cell Migration
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