ERCC8 antibody (Internal Region)

Catalog No. ABIN2857082

The Rabbit Polyclonal anti-ERCC8 antibody has been validated for WB, IF, ICC and Func. It is suitable to detect ERCC8 in samples from Human and Mouse.

Quick Overview for ERCC8 antibody (Internal Region) (ABIN2857082)

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Functional Studies (Func)

Product Details anti-ERCC8 Antibody

Binding Specificity: Internal Region

Characteristics: Rabbit Polyclonal antibody to ERCC8 (excision repair cross-complementing rodent repair deficiency, complementation group 8)
ERCC8 antibody [N2C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ERCC8. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Functional Assay Assay-dependent dilution Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000* Functional AssayAssay-dependent dilution

Comment:

Positive Control: 293T , A431 , Neuro2A , GL261 , C8D30 , NIH-3T3, Raw264.7 , C2C12

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ERCC8

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Alternative Name: ERCC8

Background: This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes.

Cellular Localization: Nucleus

Molecular Weight: 44 kDa

Gene ID: 1161

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus